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Study on the Isozyme Variability and Activity of Protein/Enzymes from Gosling Blood Infected with Goose Parvovirus
Authors:ZHU Xin-chan  WANG Qian-wen  ZHU Feng-wei  LI Hong-qiang
Affiliation:1. Key Laboratory of Plant Biotechnology in University of Shandong Province, College of Life Science, Qingdao Agriculture University, Qingdao 266109, China;2. Jimo Bureau of Animal Husbandry and Veterinary, Jimo 266200, China
Abstract:In order to understand the pathogenesis of GPV (goose parvovirus),and explore the molecular variation of protein/enzyme,the protein,protective enzymes (Est,POD,SOD,protease) and isozyme from goslings blood infected with GPV were analyzed by biochemistry and molecular biology.The results showed that the activity of protease,content of protein,the activities of POD,SOD and Est (in plasma and blood corpuscle) from goslings infected with GPV were 1.46 and 1.63,1.51 and 1.49,1.50 and 1.14,1.36 and 1.73,1.30 and 1.53 times than that of control group,respectively.In the plasma of goslings infected with GPV,2 bands (134 and 239) of SOD isozyme appeared,2 fast-zone enzyme band appeared and 2 slow-zone enzyme band deleted in Est isozyme,1 slow-zone zymoprotein band (304) deleted and 4 new main protein bands (131,86,43 and 33 ku) appeared.In the blood corpuscle of goslings infected with GPV,2 new enzyme bands (93 and 203) of POD isozyme,1 medium-zone enzyme band (160) of SOD isozyme,1 fast-zone enzyme band of Est isozyme,2 slow-zone zymoprotein band (223 and 330) and 4 new main protein bands (144,104,58 and 53 ku) appeared.These results indicated that the interaction of GPV stress and host protein/enzymes and isozyme gene expression would result in the biochemical characteristics variation of activity and isozyme patterns of protein/enzymes,and directly or indirectly affect metabolic approach and pathological biochemical function on goslings infected with GPV,and enhance the defense and stress ability of GPV.Therefore,protein/enzymes and isozyme might be sensitive enzyme of GPV infection stress,and were effective marker of pathological mechanism on investigation of virus genotype interaction with genetic susceptibility of the host.
Keywords:gosling  goose parvovirus  protein/enzymes  protective enzyme  isozyme  
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