禽呼肠孤病毒σB和σC蛋白二级结构及其细胞抗原表位预测

研究旨在运用DNAStar生物软件及Garnier-Robson、Chou-Fasman等方法分析禽呼肠孤病毒（avian reovirus，ARV）标准毒株S1133重要结构蛋白σB和σC的二级结构及其T细胞和B细胞抗原表位。通过分析其亲水性、柔韧性、表面可及性和抗原指数等特性，对σB和σC蛋白的B细胞抗原表位进行预测；以Rothbard-Taylor和AMPHI方法预测σB和σC蛋白的T细胞抗原表位。结果显示，σB和σC蛋白形成α-螺旋结构的能力较差，但与σB蛋白相比，σC蛋白含有较多的β-折叠结构、转角及无规则卷曲等二级结构，因而σC蛋白二级结构较复杂。分析结果还表明σB和σC蛋白均含有潜在B细胞和T细胞抗原表位，尤其是σC蛋白具有较多的B细胞和T细胞抗原表位。本试验为深入研究σB和σC蛋白的免疫学特性及研发ARV新型疫苗和药物靶标奠定基础。

Prediction of Secondary Structure and B-and T-cell Epitopes of σB and σC Proteins of Avian Reovirus

To predict the secondary structures and B-and T-cell epitopes of σB and σC proteins of avian reovirus（ARV),the secondary structures of ARV σB and σC proteins were predicted by DNAStar software and the Garnier-Robson,Chou-Fasman methods.B-and T-cell cell epitopes of σB and σC proteins were predicted by mutable parameters,including hydrophilicity,flexibility,surface probability and antigenicity.The analysis results showed that σB and σC proteins had few α-helix regions,meanwhile,compared with σB protein,σC protein possessed rather much β-sheet and turn regions,indicating σC protein exhibited more complex secondary structure.Furthermore,both σB and σC proteins had a few promising B cell and T cell epitopes,which would contribute to further explore the immune functions of σB and σC proteins,and provided a basis for development of ARV new type vaccine and therapeutic drugs.