Over-expression of GSTP1 increases oxaliplatin-induced apoptosis in human hepatoma HepG2 cells |
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Authors: | LIU Run-tian AN Cong-jing BAI Yun ZHENG Jian-xing |
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Institution: | 1. Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Hebei Medical University, Shijiazhuang 050000, China;
2. Physical Examination Center, The Second Affiliated Hospital of Hebei Medical University, Shijiazhuang 050000, China;
3. Department of Gastroenterology, Hebei General Hospital, Shijiazhuang 050057, China;
4. Department of Hepatobiliary Surgery, Tangshan Gongren Hospital, Tangshan 063000, China |
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Abstract: | AIM: To investigate the effect of GSTP1 over-expression on the sensitivity of human hepatoma HepG2 cells to oxaliplatin (OXA). METHODS: Adenovirus carrying GSTP1 (Ad-GSTP1) was used to infect HepG2 cells for establishing the cell line over-expressing GSTP1. The cells were randomly divided into 6 groups:control, vehicle, Ad-GSTP1, OXA, OXA+vehicle and OXA+Ad-GSTP1. The cell survival rates were examined by CCK-8 assay, and the apoptotic rates were analyzed by flow cytometry. The protein levels of GSTP1, Akt, mTOR, p-Akt and p-mTOR were determined by Western blot. RESULTS: OXA decreased the cell survival rate in a dose-dependent manner (P<0.05). The protein expression of GSTP1 increased after transfection with adenovirus. At basal level, up-regulation of GSTP1 significantly decreased the cell survival rate, increased the cell apoptosis, and inhibited the phosphorylation levels of Akt and mTOR (P<0.05). Moreover, GSTP1 over-expression enhanced the effect of OXA on the cell viability, cell apoptosis, and further inhibited the phosphorylation levels of Akt and mTOR (P<0.05).CONCLUSION: Over-expression of GSTP1 augments the enhanced effect of OXA on HepG2 cell apoptosis, which may be related to the inactivation of Akt/mTOR signaling pathway. |
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Keywords: | GSTP1 Oxaliplatin Apoptosis HepG2 cells |
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