| 死亡谷芽孢杆菌NBIF-001防治灰霉病研究 |
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| 引用本文： | 黄大野,周婷,姚经武,刘晓艳,曹春霞,杨妮娜,胡洪涛,龙同,杨自文.死亡谷芽孢杆菌NBIF-001防治灰霉病研究[J].中国蔬菜,2016,1(10):63-66. |
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| 作者姓名： | 黄大野 周婷 姚经武 刘晓艳 曹春霞 杨妮娜 胡洪涛 龙同 杨自文 |
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| 作者单位： | 1.湖北省生物农药工程研究中心，湖北武汉430064；;2. 武汉商贸职业学院，湖北武汉430205 |
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| 摘 要： | 为明确死亡谷芽孢杆菌NBIF-001菌株对灰霉病的防治效果,采用平板对峙结合显微观察确定其对灰霉病菌的抑菌活性,并采用离体组织试验研究其对黄瓜叶部灰霉病及贮藏期番茄果实灰霉病的防治效果。结果表明,在平板对峙条件下NBIF-001对灰葡萄孢具有良好的抑菌活性,显微镜下观察到菌丝发生扭曲、肿胀和变形;离体试验结果表明NBIF-001对灰霉病具有良好的生防效果,对黄瓜叶片灰霉病和番茄果实灰霉病的防效分别达到85.91%和72.52%。
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| 关 键 词： | 死亡谷芽孢杆菌 NBIF-001 菌株 灰霉病 生防效果 |
Studies on Controlling Effect of Bacillus vallismortis NBIF-001 on Gray Mold |
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| HUANG Da-ye,ZHOU Ting,YAO Jing-wu,LIU Xiao-yan,CAO Chun-xia,YANG Ni-na,HU Hong-tao,LONG Tong,YANG Zi-wen.Studies on Controlling Effect of Bacillus vallismortis NBIF-001 on Gray Mold[J].China Vegetables,2016,1(10):63-66. |
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| Authors: | HUANG Da-ye ZHOU Ting YAO Jing-wu LIU Xiao-yan CAO Chun-xia YANG Ni-na HU Hong-tao LONG Tong YANG Zi-wen |
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| Institution: | 1.Biopesticide Engineering Research Center of Hubei Province，Wuhan 430064，Hubei，China；;2.Wuhan Trade Vocational;
College，Wuhan 430205，Hubei，China |
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| Abstract: | In order to evaluate control effect of Bacillus vallismortis on gray mold of antagonistic bacterium
strain NBIF-001，this paper determined its inhibition activity to the pathogen of Botrytis cinerea by dual culture
method combined with optical microscope observation；and studied its controlling effects on cucumber leaf grey
mold and tomato fruit grey mold by in vitro tissue test．The results showed that the strain NBIF-001 had high
inhibition activity in dual culture．Optical microscope study revealed deformation of hyphal and enlargement
of cytoplasmic vacuoles．In vitro tissue test shew that NBIF-001 exhibited better biological control effect on
cucumber and tomato gray mold．The control effect reached 85.91% and 72.52%， respectively． |
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| Keywords: | Bacillus vallismortis NBIF-001 Gray mold Biological control effect |
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