Study on Proliferation Technology of Chicken Infectious Bursal Disease Virus BJQ902 Strain in DF-1 Cell Line

In order to produce high titers of infectious bursal disease virus(IBDV) antigen,the proliferation technology of chicken IBDV BJQ902 strain in DF-1 cell line was studied by the selection and optimization of the following five culture conditions,including the amount of inoculated virus,harvest time,inoculation time,culture temperature and serum concentration in maintenance media.The results showed that the optimal proliferation conditions of IBDV BJQ902 strain in DF-1 cell line were obtained as follow:The culture temperature was 37℃,the inoculum concentration was between 0.01% and 0.1% (V/V),the inoculation time was between 48 and 72 h after cell passage,the serum concentration in maintenance media was between 1% and 2%,the harvest time was between 60 and 72 h after inoculation.Under the optimal conditions,the virus titers were between 10^8.3 and 10^8.7 TCID₅₀/0.1 mL.