Cloning and Bioinformatics Analysis on CDS of Oct-1 Gene in Mouse

In order to explore the biological characteristics of Oct-1 gene in mouse,CDS region of Oct-1 gene in mouse was cloned.And the sequence,physicochemical property,subcellular localization,target genes and conservative structure domain of Oct-1 gene were analyzed by bioinformatics method to predict whether the Oct-1 gene could regulate the production of melanin.The results showed that the length of Oct-1 gene in mouse was 2 313 bp.The formula of Oct-1 protein was C₃₄₂₅H₅₆₀₆N₉₇₆O₁₁₆₃S₁₅,which was an unstable and soluble protein.The secondary structure of Oct-1 mainly contained random coil and α-helices.Subcellular localization of Oct-1 was mainly in the nucleus,which Oct-1 might play a role of signal transducer or transcription regulation in the energy metabolism and cofactor biosynthesis.The Oct-1 gene regulated the expression of 13 genes associated with the melanin formation of mouse.The protein was encoded by Oct-1 gene including a homeo-domain and a POU domain.There were binding sites of Oct-1 on the promoters of MITF,TYR,TYRP1 and TYRP2. Oct-1 might interact with Brf2,Pou2af1 and Tbp to regulate the colour gene expression.We inferred that the transcription factor Oct-1 played an important role in melanogenesis and these results provided a theoretical basis for studying the coat colour formation mechanism.