The Optimization of Preparating Conditions on Pig ICSI Embryos

In order to optimize the preparating conditions on pig intracytoplasmic sperm injection (ICSI) embryos,the Piezo operating system was used in this experiment and the embryonic survival rate,cleavage rate and cell numbers of blastocysts developed in various stages were measured as traditional and Piezo operating system,the concentration of CB,the operating temperature with Piezo operating system and different sperm processing method in preparating pig ICSI embryos.The results showed that Piezo operating system could significantly improve the survival rate and cleavage rate of injected oocytes (P<0.05),but there was no significant effect on blastocyst rate.There was a slight damage to oocyte added CB in the operating fluid,and adding 7.5 μg/mL CB was most concentration,30℃ was most appropriate operating temperature,but there was no significant effect of early development on pig ICSI embryos with breaking sperm tail with ultrasonic wave.The results suggested that Piezo operating system could significantly improve the survival rate.The results suggested that breaking sperm tail with ultrasonic wave,adding 7.5 μg/mL CB in operating fluid and 30℃ of operating temperature and using Piezo operating system was more suitable for preparating pig ICSI embryos.