番茄自交系YNAU335对番茄斑萎病毒抗性的鉴定

番茄斑萎病毒(Tomato spotted wilt virus,TSWV)已成为近年来危害番茄生产的重要病害,鉴定和选育抗病品种是防治TSWV的有效途径。选取自主培育和引进的番茄品种(系),经过连续3a的大棚蓟马传毒和光照培养箱人工接种试验,鉴定番茄品种(系)对TSWV的抗性。结果表明,番茄自交系YNAU335对TSWV表现为免疫,其他品种全部表现为感病,而且在YNAU335人工接种和大田蓟马传毒叶片中并未克隆出TSWV核衣壳蛋白基因和小片段RNA3′端序列,而在其他感病品种(系)中均克隆出这2段序列。研究结果初步确定番茄自交系YNAU335为TSWV抗病品系,可作为TSWV抗病育种的番茄资源。

Resistance Identification of Tomato Inbred Line‘ YNAU335’ to TSWV

Recently，Tomato spotted wilt virus（TSWV）has become a serious disease hazarding tomato production. Identification and selective breeding of disease-resistant varieties is an effective approach to control TSWV. This experiment selected tomato varieties（lines）bred by our group and introduced from other countries，through virus transmission by thrips in greenhouse，and artificial inoculation in illumination incubator for 3 consecutive years to identify the disease resistance against TSWV. The results indicated that inbred line‘ YNAU335’ was immune to TSWV，while the other varieties were all susceptible to TSWV. Furthermore，the nuclear protein gene and small RNA 3′ flank sequences of TSWV in leaves of ‘YNAU335’ were not cloned. On the contrary，these 2 sequences were cloned in other tomato leaves. The results preliminarily demonstrated that‘ YNAU335’ was resistant to TSWV，and could be used as disease resistant tomato resource in resistant breeding against TSWV .