Isolation,Identification and Drug Sensitive Tests of Porcine Contagious Actinobacillus pleuropneumoniae

In order to provide a scientific prevention and treatment of porcine contagious pleuropneumia (PCP) disease in a pig farm of Tianjin,pigs with suspected PCP were carried on clinical comprehensive diagnosis and histopathological observations.A systematic analysis of isolated strain were conducted by laboratory diagnostic methods included bacterial isolation and culture,microscopic examination,biochemical tests,satellite phenomenon examination,PCR detection,animal pathogenicity experiments and drug sensitive tests.The most sick pigs were dyspnea,and the typical results of pathological anatomy and histopathological observations were fibrinous pneumonia.The gram-negative and polymorphous bacilli could be seen under the microscopy,its biochemical characteristics and satellite phenomenon were consistent with those of Actinobacillus pleuropneumoniae(APP).The amplified result of APP APX Ⅳ A gene was positive by specific primers.The isolated strain could be lethal to mice and it was highly sensitive to cefotaxime and florfenicol.Combined with the results of laboratory detection and the clinical comprehensive diagnosis,it was confirmed the isolated strain was APP.