Relationship of CTCs with CD4+/CD8+, NLR,TTV and tumor stage in patients with primary hepatocellular carcinoma |
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Authors: | WAN Lei CHEN Jia-jia LIU Jian-jun KAN He-ping |
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Affiliation: | Department of Hepatobiliary Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China |
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Abstract: | AIM: To explore the relationship of circulating tumor cells (CTCs) with CD4+/CD8+, neutrophil-to-lymphocyte ratio (NLR), total tumor volume (TTV) and tumor stage in the patients with primary hepatocellular carcinoma.METHODS: We selected 80 cases of histologically diagnosed primary hepatocellular carcinoma in the study. The method of CanPatrolTM was used, which was developed by SurExam biotechnology company to identify CTCs in the blood. CD4+ T cells and CD8+ T cells were counted by flow cytometry. The patients were divided into 2 groups according to the levels of CD4+/CD8+ ratio, NLR and TTV. The patients were also divided into Ⅰ+ Ⅱstage group and Ⅲ+ Ⅳ stage group according to the seventh edition of TMN staging criteria.RESULTS: The numbers of peripheral blood CTCs and mesenchymal CTCs in high CD4+/CD8+ ratio group were significantly lower than those in low CD4+/CD8+ ratio group (P < 0.05). The numbers of peripheral blood CTCs, mesenchymal CTCs and hybrid CTCs in high TTV group were significantly higher than those in low TTV group (P < 0.05). The numbers of peripheral blood CTCs, mesenchymal CTCs and hybrid CTCs in I+ II stage group were significantly lower than those in III+ IV stage group (P < 0.05). The numbers of peripheral blood CTCs, mesenchymal CTCs, hybrid CTCs and epithelial CTCs between high NLR group and low NLR group had no statistical difference.CONCLUSION: CTCs exist in the peripheral blood of the patients with primary hepatocellular carcinoma. The peripheral blood CTCs have significant correlations with TTV, tumor stage and T-cell immunity.The worse cell immune function, the larger TTV and the later tumor stage a patient has, the more the peripheral blood CTCs are. |
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Keywords: | Hepatocellular carcinoma Circulating tumor cells T-cell immune Total tumor volume Neutrophil-to-lymphocyte ratio Tumor stage |
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