Effect of MG132 on Aβ generation in SH-SY5Y cells

AIM: To observe the influences of different concentrations of MG132 on apoptosis and beta-amyloid protein(Aβ) generation in SH-SY5Y cells, and to explore the underlying mechanism.METHODS: SHSY-5Y cells were incubated with MG132 for 24 h. The final concentrations of MG132 were 2.5, 5 and 10 μmol/L. The cell viability was determined by MTT assay. The cell apoptosis was assessed by flow cytometry. The levels of Aβ were measured by ELISA. The relative protein levels were detected by Western blot.RESULTS: In the SH-SY5Y cells, MG132 reduced the cell viability, induced the cell apoptosis, increased the level of Aβ, and increased the expression of the related proteins for Aβ generation in a concentration-dependent manner.CONCLUSION: MG132 induces apoptosis and increases the levels of Aβ_1-42 and Aβ_1-40 by regulating the proteins related to Aβ generation in the SH-SY5Y cells.