Cloning and Bioinformatics Analysis of MAP3K5 Gene CDS Sequence in Pig

In this study,we cloned MAP3K5 gene cDNA sequence,compared the sequence of MAP3K5 with MAP3K families in pig and other species,and studied their conservative structure domain.Obtained the full-length of MAP3K5 gene (5 452 bp) by RACE-PCR,predicted the open reading frame (ORF) and amino acid (AA) sequence,and obtained the information of 31 exons.The low similarity were found in mRNA and amino acid sequences of MAP3K families in pig (<50%).And MAP3K5 had the comparatively high similarity with MAP3K6 in pig.The comparative analyzed the mRNA and protein sequence of pig MAP3K5 gene with other species MAP3K5 gene,founded that the ten species of MAP3K5 mRNA and protein sequence had the high similarity (>78%),and pig MAP3K5 had the comparatively high similarity with Bos taurus,Ovis aries,Canis lupus and Homo sapiens.Function domain analyzed the high similarity protein with MAP3K5 in pig,this research found that pig MAP3K6 protein had the similar conserved function domain with pig MAP3K5.However,the other pig MAP3K families didn't have the similar conserved function domain with pig MAP3K5.The ten species of MAP3K5 protein had the similar conserved function domain.The results obtained the sequence and molecular structure of MAP3K5 gene,and provided an important foundation for further function research of MAP3K5 gene in pig.