Effect of Sonic Hedgehog signaling blockade on growth of hepatocarcinoma cells |
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Authors: | LIU Ai-mei YU Gong-wang HUANG Li-xia SUN Yan CHI Zuo-hua |
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Affiliation: | 1. School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China;2. Medical College, Guangdong Pharmaceutical University, Guangzhou 510006, China |
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Abstract: | AIM: To investigate the effect of Sonic Hedgehog (Shh) signaling blockade on the growth of hematocarcinoma cells and underlying mechanisms. METHODS: The expression of Shh signaling molecules in hematocarcinoma cell lines BEL-7402, Huh7 and HepG2 was detected by RT-PCR. The cell viability was detected by MTT assay. The cell cycle and apoptosis were analyzed by flow cytometry. The expression of apoptosis-related proteins was determined by Western blot. RESULTS: Shh signaling molecules were all expressed in BEL-7402, Huh7 and HepG2 cells. The mRNA expression of Patched (Ptch), Gli1 and Gli2 was down-regulated by anti-Shh antibody. Blockade of Shh signaling pathway inhibited the proliferation of hepatocarcinoma cells with increasing cells in G0/G1 phase and induced the apoptosis of hepatocarcinoma cells. Treatment with anti-Shh antibody down-regulated the protein expression of pro-caspase-3, pro-caspase-8 and pro-caspase-9, while up-regulated the protein levels of cleaved caspase-3, cleaved caspase-8 and cleaved caspase-9 in BEL-7402 cells. CONCLUSION: Blockade of Shh signaling pathway inhibits the growth of hepatocarcinoma at different levels by cell cycle arrest and inducing apoptosis of hematocarcinoma cells. |
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Keywords: | Hepatocarcinoma cells Sonic Hedgehog Apoptosis |
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