Effect of MSTN Gene Upstream Sequence Polymorphism on Transcriptional Expression in Luxi Huang Cattle |
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| Authors: | WANG Dan-dan LIU Xiao-mu ZHANG Ran LIU Gui-fen WAN Fa-chun LIN Yu-shuang |
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| Affiliation: | 1. Provincial Key Laboratory of Animal Cells and Developmental Biology, Life Science College, Shandong University, Jinan 250100, China;2. Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veternary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China |
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| Abstract: | Myostain (MSTN) gene is a member of transforming growth factor-β (TGF-β) superfamily.In order to study the relationship between Luxi Huang cattle MSTN gene upstream sequence polymorphism and the transcriptional expression,DNA was extracted from leg muscle cells of Luxi Huang cattle and then sequenced.After analyzing MSTN upstream sequence, an evolutionary tree was constructed and a SNP site was found at 805 bp of MSTN upstream sequence of initiation codon.Two expression vectors contained the SNP site were constructed to transfect the C2C12 cells in vitro to study the influence of MSTN gene upstream sequence polymorphism on transcription activity.The results showed that single nucleotide change on MSTN gene upstream sequence affected the expression.It suggested that MSTN gene upstream sequence polymorphism of Luxi Huang cattle would affect transcription activity and play an important role in gene expression regulation. |
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| Keywords: | MSTN gene upstream sequence single nucleotide polymorphism transcriptional expression |
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