| 牛血清中蛋白组分初步分析及其对细胞培养的影响 |
| |
| 引用本文： | 张宝红,张为宇,韩忠燕,黄维义,钟恒禄,李健. 牛血清中蛋白组分初步分析及其对细胞培养的影响[J]. 动物医学进展, 2009, 30(1) |
| |
| 作者姓名： | 张宝红 张为宇 韩忠燕 黄维义 钟恒禄 李健 |
| |
| 作者单位： | 广西大学动物科学技术学院,广西,南宁,530005;广西大学动物科学技术学院,广西,南宁,530005;广西大学动物科学技术学院,广西,南宁,530005;广西大学动物科学技术学院,广西,南宁,530005;广西大学动物科学技术学院,广西,南宁,530005;广西大学动物科学技术学院,广西,南宁,530005 |
| |
| 基金项目： | 南宁市市校科技合作专项基金，广西大学博士科研启动基金 |
| |
| 摘 要： | 为探讨牛血清中蛋白组分对细胞培养的影响,应用凝胶过滤层析对不同牛血清进行初步组分分析,并应用MTT比色法衡量不同牛血清的促细胞生长作用.结果表明,蛋白组分大小相对较为一致的新生牛血清、商品血清1、2、5(一个波峰)与蛋白组分差异较大的成年牛血清、商品血清3、4(两个或者两个以上波峰)的促细胞生长作用相比较差异显著(P
|
| 关 键 词： | 牛血清 蛋白组分 细胞培养 |
A Preliminary Analysis of the Protein Compositions of Bovine Serum and Its Effect on Cell Culture |
| |
| ZHANG Bao-hong,ZHANG Wei-yu,HAN Zhong-yan,HUANG Wei-yi,ZHONG Heng-lu,LI Jian. A Preliminary Analysis of the Protein Compositions of Bovine Serum and Its Effect on Cell Culture[J]. Progress In Veterinary Medicine, 2009, 30(1) |
| |
| Authors: | ZHANG Bao-hong ZHANG Wei-yu HAN Zhong-yan HUANG Wei-yi ZHONG Heng-lu LI Jian |
| |
| Affiliation: | College of Animal Science and Technology;Guangxi University;Nanning;Guangxi;530005;China |
| |
| Abstract: | In order to investigate the cell culture which was influenced by protein compositions of bovine serum,this work was to make a preliminary anlysis of the protein compositions of bovine serum by the method of gel filtration chromatography(GFC) and to measure the effects of promoting the growth and proliferation of cell that using different bovine serum by the MTT assay.The results showed that the serum contains the relatively uniform protein compositions which were one peak through the GFC(newborn bovine seru... |
| |
| Keywords: | bovine serum protein components cell culture |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
