屠宰生猪体内猪链球菌2型带菌情况调查

应用猪链球菌2型定型PCR方法，对从福建省3个屠宰场采集的临床健康的生猪鼻咽拭子样本共121份进行了检测，同时对阳性样本进行了细菌分离鉴定及毒力基因检测。结果显示：121份样本中有17份为阳性，检出率为14．0％（17／121），其中福州地区检出率为10．9％（7／64），宁德地区检出率为11．8％（4／34），莆田地区检出率为26．1％（6／23）。3株分离菌cps2J基因PCR特异性扩增产物大小均为675bp，其核苷酸序列与Genebank中猪链球菌2型参考菌株的同源性高达98％～100％，毒力基因型为gdh^＋／cps2J^＋／mrp^＋／ef^-／sly^-／fbps^＋／orf2^＋。调查结果表明，福建省福州、宁德、莆田等地区生猪群中均存在猪链球菌2型携带者，所分离到的3个猪链球菌2型菌株其毒力基因型为新的基因型。

Carriers of Streptococcus suis type 2 among slaughtered pigs in Fujian

One-hundred-twenty-one nasopharyngeal swab samples of slaughtered,clinically healthy pigs from 3 slaughter houses in different areas of Fujian were collected for isolation of Streptococcus suis type 2(SS2) and detection of the virulence genotype with specific PCR.The results showed a total positive rate of SS2 of 14.0%(17/121) among the pigs sampled.The positive rates in Fuzhou,Ningde and Putian were 10.9%(7/64),11.8%(4/34) and 26.1%(6/23),respectively.The PCR amplified products of the cps2J gene from 3 iso...