大杯蕈原生质体菌株筛选的研究

对野生大杯蕈（Ｃｌｉｔｏｃｙｂｅ ｍａｘｉｍａ）原生质体的分离再生及再生菌株的构建进行研究，以纤维素酶、溶壁酶、崩溃酶、β－葡萄糖苷酸酶的去壁效果最好，而新生酶，几丁质酶，蜗牛酶的云壁效果较差。２种以上的酶液混合使用能提高去壁效果。酶解液在０．１％￣２．０％浓度范围内原生质体释放量随酶解液的浓度升高几乎呈直线上升。酶解３￣５ｈ释放的原生质体最多。酶解液ｐＨ值在５．０￣６．０范围内原生质体的产量较高

A Study on the Construction of Protoplast Regenerating Strains in a Wild Mushroom Clitocybe maxima

AIM:To study the changes of β-adrenoceptors in peripheral lymphocytes at different stages of asthma. METHODS:β-adrenoceptors in peripheral lymphocytes, cAMP and cGMP levels in blood plasma were measured by radioligand binding assay and radioimmunoassay. RESULTS: Bmax and Kd of β-adrenoceptors in asthma at remission stage were markedly higher than that in normal subjects, while cAMP levels in blood plasma were not different between them. Bmax of β-adrenoceptors and cAMP levels in blood plasma in asthma at acute exacerbation stage were significantly lower than that in normal subjects, Kds between these two groups were not much different.There was no correlation between P-adrenoceptors, cAMP and cGMP levels in blood plasma and ventilatory funs dons.CONCLUSIONS:Changes of β-adrenoceptors in asthma rather be a pathological change accompanied by the course of the disease than a primary defect.