纳米氧化锌对肉仔鸡抗氧化性能的影响

本试验旨在研究纳米氧化锌对肉仔鸡抗氧化性能的影响.选用1日龄爱拔益加肉仔鸡192只,随机分为4个组,每个组4个重复,每个重复12只鸡.以添加硫酸锌(含锌80 mg/kg)的口粮为对照组,即A组,添加含锌40、80、120 mg/kg的3个纳米氧化锌水平组为试验组,即B、C、D组,试验期6周.试验结果表明:40 mg/kg锌添加水平的纳米氧化锌组能够显著提高(21、42 d)肉仔鸡血清中谷胱甘肽过氧化物酶(GSH-Px)活性(P<0.05、P<0.01),极显著提高(21、42 d)血清中总抗氧化能力(T-AOC)(P<0.01、P<0.01),减少(21 d)血清中一氧化氮(NO)生成(P<0.05).80 mg/kg锌添加水平的纳米氧化锌组能够极显著提高(42 d)肉仔鸡血清中GSH-Px活性(P<0.01),显著提高(42 d)肝组织中过氧化氢酶(CAT)和T-AOC活性(P<0.05、P<0.01),极显著提高(21 d)血清中T-AOC(P<0.01),减少(21、42 d)血清中NO生成(P<0.05、P<0.01),并能提高(42 d)血清抑制羟自由基能力(P<0.05),提高(21 d)肝组织中抗超氧阴离子自由基活性(P<0.05).由此可知,日粮添加纳米氧化锌可在一定程度上提高肉仔鸡机体的抗氧化性能,增强抗氧化酶活性,降低自由基的产生,以40、80 mg/kg添加量效果较佳.

Effects of Nano-zinc Oxide on Antioxidant Function in Broilers

The experiment was conducted to study the effects of nano-zinc oxide on antioxidant function in broilers.One hun-dred and ninety-two Arbor Acres (AA) broilers at the age of one day old were randomly divided into 4 groups with 4 repli-cates in each group and 12 birds in each replicate.The zinc sulfate (zinc level of 80 mg/kg) supplemented in diet was the control group (group A).Zinc supplemental levels of 40, 80, 120 mg/kg were groups B, C and D, which were added as nano-zinc oxide.The experiment lasted for 6 weeks.The results were as follows: supplemental Zn level of 40 mg/kg, the activity of GSH-Px in serum (21, 42 d) of broiler were significantly increased (P<0.05, P<0.01), the T-AOC in serum (21, 42 d) were significantly increased (P<0.01, P<0.01), the content of MDA in serum (21 d) were reduced (P< 0.05) ; Zn supplemental level of 80 mg/kg, the activity of GSH-Px (42 d) was significantly increased (P<0.01), the CAT and T-AOC in liver (42 d) were significantly increased (P<0.05, P<0.01), the activity of T-AOC in serum (21 d) was significantly increased (P<0.05), the content of NO in serum (21, 42 d) were reduced (P<0.05, P<0.01), and en-hanced the ability of inhibiting hydroxyl radical (·OH) in serum (42 d) (P<0.05), also enhanced the activity of resisting superoxide anion free radical (O2-·) in liver (21 d) (P<0.05).The results showed that adding protein-bound zinc and nano-zinc oxide to diet could enhance antioxidant function in broilers, strengthen the activities of antioxidases and reduce the levels of free radicals, in which supplemental nano-zinc oxide level of 40, 80 mg/kg were optimal levels.