| 防治青枯病工程菌Hrp-菌株的发酵培养基配方优化 |
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| 引用本文： | 冉淦侨,朱昌雄,田云龙,郭萍,黄亚丽,肖晶晶.防治青枯病工程菌Hrp-菌株的发酵培养基配方优化[J].中国生物防治,2010(1):73-79. |
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| 作者姓名： | 冉淦侨 朱昌雄 田云龙 郭萍 黄亚丽 肖晶晶 |
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| 作者单位： | 1. 中国农业科学院农业环境与可持续发展研究所,北京100081;
2. 河北省科学院生物研究所,石家庄050011; |
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| 基金项目： | 国家“948”项目(2006-G54) |
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| 摘 要： | 在摇瓶发酵条件下,采用全因子实验设计法和正交实验设计法对工程菌Hrp-菌株的培养基碳、氮源配方进行优化,确定了最适碳源为葡萄糖、细玉米面,最适氮源为硫酸铵、细豆饼粉;对这四个碳氮源因子的浓度进行L9（34）的正交试验,最终确定出该菌的最适发酵培养基碳、氮源配方为（g/L）：葡萄糖8g、细玉米面5g、细豆饼粉35g、硫酸铵2g。优化后的培养基配方使摇瓶发酵液菌量从1.34×10^10cfu/ml提高至3.30×10^10cfu/ml。
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| 关 键 词： | 青枯病 工程菌 Hrp-菌株 发酵培养基 碳氮源 优化 |
Optimization of Carbon and Nitrogen Sources for fermentation of Hrp~- Mutant of Ralstonia solanacearum as Biocontrol Agent of Bacterial Wilt |
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| RAN Gan-qiao
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ZHU Chang-xiong
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TIAN Yun-long
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GUO Ping
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HUANG Ya-li
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XIAO Jing-jing.Optimization of Carbon and Nitrogen Sources for fermentation of Hrp~- Mutant of Ralstonia solanacearum as Biocontrol Agent of Bacterial Wilt[J].Chinese Journal of Biological Control,2010(1):73-79. |
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| Authors: | RAN Gan-qiao ZHU Chang-xiong TIAN Yun-long GUO Ping HUANG Ya-li XIAO Jing-jing |
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| Institution: | RAN Gan-qiao1,ZHU Chang-xiong1,TIAN Yun-long1,GUO Ping1,HUANG Ya-li2,XIAO Jing-jing1(1.Institute of Environment , Sustainable Development in Agriculture,CAAS,Beijing 100081,2.Institute of Biology,Hebei Academy of Science,Shijiazhuang 050011,China) |
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| Abstract: | Optimization of carbon and nitrogen sources for fermentation of Hrp-mutant of Ralstonia solanacearum was conducted applying full factorial design and orthogonal analysis under the condition of flask fermentation.The best carbon sources were glucose and fine corn flour.The best nitrogen sources were(NH4)2SO4 and bean cake powder.Based on the orthogonal test,the best formulation of carbon and nitrogen sources was gained,which was glucose 8 g,fine corn flour 5 g,bean cake powder 35 g and(NH4)2SO4 2 g in 1 L me... |
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| Keywords: | bacterial wilt genetically modified bacteria Hrp-strain fermentation medium carbon and nitrogen sources optimization |
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