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普遍适用于农作物基因组DNA的提取方法及提取值得注意的几个问题
引用本文:朱英,陶刚,刘作易,宋吉轩. 普遍适用于农作物基因组DNA的提取方法及提取值得注意的几个问题[J]. 贵州农业科学, 2005, 33(5): 20-21
作者姓名:朱英  陶刚  刘作易  宋吉轩
作者单位:贵州省农业生物技术重点实验室,贵阳550006;贵州省农业科学院,生物技术研究所,贵阳,550006;贵州省农业生物技术重点实验室,贵阳550006;贵州省农业科学院,生物技术研究所,贵阳,550006
基金项目:贵州省科技攻关项目[黔科合农社会字(2002)1136号]
摘    要:介绍了一种普遍适用于水稻、玉米、马铃薯、油菜、小麦等农作物材料基因组DNA的提取方法,并就在提取过程中遇到的问题,如RNA去除不干净,蛋白质或酚残留、DNA色素较深以及DNA机械剪切严重等,提出了相应的解决办法.

关 键 词:农作物  基因组DNA  提取方法
文章编号:1001-3601(2005)05-0237-0020-02
收稿时间:2005-05-16
修稿时间:2005-05-16

A Common Method for Genomic DNA Extraction from the Main Crops and Several Problems of being Noticed in Extraction Practice
ZHU Ying,TAO Gang,LIU Zuo-yi,SONG Ji-xuan. A Common Method for Genomic DNA Extraction from the Main Crops and Several Problems of being Noticed in Extraction Practice[J]. Guizhou Agricultural Sciences, 2005, 33(5): 20-21
Authors:ZHU Ying  TAO Gang  LIU Zuo-yi  SONG Ji-xuan
Affiliation:1. The Key Laboratory for Agricultural Biotechnology of Guizhou, Guiyang 550006; 2. Institute of Biotechnology , Guizhou Academy of Agricultural Sciences, Gui yang 550006 ; 3. Institute of Plant Protection, Guizhou Academy of Agricultural Sciences, Guiyang 550006; 4. Guizhou Academy of Agricultural Sciences, Guiyang 550006, China
Abstract:In this paper, a common method for extraction of genomic DNA from the important crops, such as rice, maize, potato, rape and wheat, was demonstrated to be feasible. And the corresponding resolvents were provided for problems which affected heavely the experimental result during the extraction.
Keywords:crop   genomic DNA   extraction method
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