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| 大萼杜鹃的快速繁殖研究 | |
| 引用本文： | 苗永美,王永清,庄平,简兴.大萼杜鹃的快速繁殖研究[J].中国林副特产,2007(4):12-14. |
| 作者姓名： | 苗永美 王永清 庄平 简兴 |
| 作者单位： | 1. 四川农业大学林学园艺学院,四川,雅安,625014;安徽科技学院生命科学学院,安徽,凤阳,233100 2. 四川农业大学林学园艺学院,四川,雅安,625014 3. 中国科学院华西亚高山植物园 4. 安徽科技学院生命科学学院,安徽,凤阳,233100 |
| 摘 要： | 大萼杜鹃的种子无菌发芽获得试管苗,然后选用试管苗的芽苗进行离体培养,并且通过正交试验设计并结合方差分析、多重比较,建立了大萼杜鹃快繁体系。试验结果表明:愈伤组织的诱导培养基是Read TDZ(0.2~0.4mg/L) NAA(0.01~0.1 mg/L);愈伤组织的再分化培养基是Read TDZ0.3 mg/L NAA0.01 mg/L AC0.2%;生根培养基是Read IBA0.25 mg/L NAA0.25 mg/L AC0.20~0.60% 蔗糖15.00~30.00g/L。 |
| 关 键 词： | 活性炭 培养基 繁殖 杜鹃 组织培养 |
Study of rapid propagation technology of Rhododendron Megacalyx |
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| Miao Yongmei,Wang Yongqing,Zhuang Ping,Jian Xing.Study of rapid propagation technology of Rhododendron Megacalyx[J].Forest By-product and Speciality in China,2007(4):12-14. | |
| Authors: | Miao Yongmei Wang Yongqing Zhuang Ping Jian Xing |
| Abstract: | The seeds of Rhododendron megacalyx Balf.f.et Ward germinated under aseptic conditions,then the shoots from Rhododendron tube seedlings were used as explants,the system of rapid propagation was established by means of the orthogonal experiment design,analysis of variance and multiple comparisons.The results were as follows :Induction medium of callus was Read TDZ(0.2-0.4mg/L) NAA(0.01-0.1 mg/L);Redifferentiation medium of callus was Read TDZ0.3 mg/L NAA0.01 mg/L AC0.2%;The rooting medium was Read IBA0.25 mg/L NAA0.25 mg/L AC0.20-0.60% sugar15.0030.00g/L. |
| Keywords: | Activated carbon Medium Propagation Rhododendron Tissue culture |
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