家兔血清中苦马豆素的气相色谱测定方法

建立家兔血清中苦马豆素(SW)浓度的气相色谱测定方法,为SW的毒理学和免疫学研究提供条件。饲喂家兔甘肃棘豆草粉后,分别于5、10、20d采集血液并分离血清;血清经过丙酮沉淀、超声和离心净化处理后,冷冻干燥;冻干物经硅烷化试剂衍生化反应,用气相色谱内标法测定血清中SW的含量。结果显示,测定SW的线性范围为0.00008～2.5g/L,相关系数γ=0.9996,检出限为0.00008g/L。在0.001、0.01、0.1、1.0g/L分别添加SW的回收率为89.81%～96.23%,相对标准偏差为2.46%～4.35%。结果表明,该方法准确、灵敏、快速、简便,适用于家兔血清中SW含量的测定。

The determination of swainsonine in rabbit serum by gas chromatography

A gas chromatographic(GC) method was established to determinate the concentration of swainsonine in the rabbits serum,which created the conditions for the next immunological study. The rabbit serum was collected in the 5 th, 10 th and 20 th day afer feeded Oxytropis kansuensis, respectively,acetone was added in the serum samples,then the Vacuum Freezy-desiccation were used after purified by ultrasonic wave and centrifuging. Then the concen-tration of swainsonine in the serum samples were analyzed by gas chromatographic internal standard method after de-rivatization with slilylreagents. The standard curve was linear within the investigated range of 0.000 08-2.5 g/L.The correlation coefficient was 0. 999 6. The detection limit was 0.000 08 g /L. The recoveries were 89.81%-96.23% with the relative standard deviations of 2.46%-4.35% at the spiking levels of 0.001-1.0 g/L. The method is accurate,sensitive,rapid and simple,it could be used to determinate the serum concentration of swainsonine in the rabbit.