接种不同剂量E.tenella对雏鸡血清抗氧化酶的影响

同一日龄雏鸡接种不同剂量的柔嫩艾美尔球虫,研究接种不同剂量柔嫩艾美尔球虫对雏鸡血清抗氧化酶的影响。将60只健康鸡随机分为6组(n=10),分别接种0、0.5×104、1×104、2×104、4×104和8×104个孢子化卵囊。接种后7 d颈静脉采血,分离血清,测定血清脂质过氧化物(MDA)含量和超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、过氧化氢酶(CAT)活性。结果表明雏鸡感染不同剂量的柔嫩艾美尔球虫后,按接种剂量从低到高,血清中MDA含量先降低后升高变化差异极显著(P0.01);血清SOD活性先升高后降低,差异极显著(P0.01);血清GSH-PX活性先升高后降低,差异极显著(P0.01);血清CAT活性较对照组极显著性降低(P0.01)。不同剂量柔嫩艾美尔球虫感染对雏鸡血清MDA含量和SOD、GSH-PX、CAT活性有一定的影响。

Effect on serum antioxidase of the same age chicken inoculated with different doses E.tenella

Inoculating the same age chicken with different doses of E.tenella to study effect on serum antioxidase.Sixty one-day-old healthy chickens,fed for 14 days in eonccidian-free condition,were chosen and randomly divided into Ⅰ,Ⅱ,Ⅲ,Ⅳ,Ⅴ,Ⅵ groups(n= 10).Chickens in Ⅰ group were not inoculated as control group ,each chicken in Ⅱ-Ⅵ groups were orally inoculated with 0,0.5×10~4,1×10~4,2×10~4 ,4×10~4,8×10~4 sporulated oocysts of E.tenel-la,respectively.All ckickens were bled from jugular vein at post-infection (14 d).The indexes such as maleic dialde-hyde(MDA).catalase(CAT).superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) in serum were de-termined.The results showed that CAT in serum declined significantly SOD and GSH-PX in serum increased signifi-cantly ,and MDA in serum decreased firstly and then increased significantly after infection with E.tenella.It is con-cluded that E.tenella of different dosage affects greatly serum antioxidase.