番鸭A-FABP基因部分序列多态性与屠宰、肉质性状和血清甘油三酯含量关系

根据家鸭A-FABPmRNA序列和鸡A-FABP基因组序列设计1对引物扩增番鸭A-FABP基因内含子1序列。同时根据扩增产物设计5对引物,利用PCR-SSCP对番鸭A-FABP基因部分进行多态性研究及多态性与部分屠宰性状、肉质性状、胸肌肌内脂肪含量和血清甘油三酯含量关系。结果显示,克隆序列包含番鸭A-FABP基因外显子1、2部分序列和完整的内含子1序列,与家鸭和鸡A-FABP基因内含子1分别有序列94.43%和72.95%的同源性;经SSCP检测,在引物P3扩增片段上发现2处碱基突变:分别为1074处T-G和1089处A-C突变,这2处突变产生3种基因型AA、BB和AB型,χ2检验显示番鸭群体符合Hardy-Weinberg平衡(P0.05)。最小二乘分析显示,AA、AB型个体在胸肌率、肌肉pH值以及胸肌肌内脂肪含量均存在显著性差异(P0.05)。结果表明,番鸭A-FABP基因内含子1多态性可能对个体生产性能产生重要的影响。

Association of the A-FABP gene polymorphism with the slaughter trait, meat quality trait and serum triglyceride in muscovy

A pair of primers P-in1 were designed according to the sequences of adipocyte fatty acid-binding protein (A-FABP) gene mRNA in duck and A-FABP gene in chicken to amplify muscovy A-FABP intron-1, then 5 pairs of primers were designed according to the cloned gene. The SNPs were detected by technique of PCR-SSCP, confirmed by sequencing, then studied the association of polymorphisms of A-FABP gene with the slaughter trait, meat quality trait and content of intramuscular fat and serum triglyceride. The results indicated that the product of primer P-in1 included partial exon-1, exon-2 and complete intron-1 after cloned and sequenced and homologous comparison with muscovy and duck, chicken A-FABP gene, was 94.43% and 75.1%. T-G at 1 074 and A-C at 1 089 nucleotide mu-tation were found in the products of primer P3, which resulted in 3 genotypes and the results of Chi-Square test showed that the groups was fit with the Hardy-Weinberg equilibrium (P>0.05). The least square analysis showed that there was significant difference (P<0.05) between AA and AB genotype birds in percentage of breast muscle,pH value and IMF. The results suggested that the polymorphisms of intron 1 of A-FABP gene have a certain effect on performance trait.