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| 甘氨酸对延边黄牛体细胞克隆重组胚发育的影响 | |
| 引用本文： | 韩岩,魏丽荣,孙丽丽,张涛,李鹏飞,王洪宝,李钟淑,方南洙.甘氨酸对延边黄牛体细胞克隆重组胚发育的影响[J].中国兽医学报,2010,30(3). |
| 作者姓名： | 韩岩 魏丽荣 孙丽丽 张涛 李鹏飞 王洪宝 李钟淑 方南洙 |
| 作者单位： | 1. 延边大学,农学院,吉林,龙井,133400 2. 延边大学,农学院,吉林,龙井,133400;首都医科大学,附属北京天坛医院药剂科,北京,100000 3. 吉林省龙井市畜牧兽医局,吉林,龙井,133400 |
| 基金项目： | 国家自然科学基金，延边大学211工程三期立项项目 |
| 摘 要： | 为了提高延边黄牛体细胞克隆重组胚的发育率,研究了在体外培养液中添加不同浓度的甘氨酸对重组胚发育的影响。分别在体外培养液中添加0、7、14、21mmol/L的甘氨酸,结果表明,在体外培养液中添加甘氨酸能够显著提高延边黄牛体细胞克隆重组胚的发育率,且以7mmol/L浓度的甘氨酸囊胚的发育率最高。 |
| 关 键 词： | 延边黄牛 体细胞克隆 甘氨酸 |
Effect of glycine on the development of somatic cell cloned reorganization embryo of Yanbian yellow cattle |
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| HAN Yan,WEI Li-rong,SUN Li-li,ZHANG Tao,LI Peng-fei,WANG Hong-bao,LI Zhong-shu,FANG Nang-zhu.Effect of glycine on the development of somatic cell cloned reorganization embryo of Yanbian yellow cattle[J].Chinese Journal of Veterinary Science,2010,30(3). | |
| Authors: | HAN Yan WEI Li-rong SUN Li-li ZHANG Tao LI Peng-fei WANG Hong-bao LI Zhong-shu FANG Nang-zhu |
| Abstract: | In order to improve the efficiency of Yanbian yellow cattle somatic cell cloned recombinant embryos development, The study of the in vitro culture medium added (0,7,14,21 mmol/L) on the impact of in vitro culture medium with different concentrations of glycine the reorganization embryo development. Adding glycine could increase the cleavage rate of embryos reorganization, but 7 mmol/L glycine displayed the highest rate of blastocyst development,decreased in the other groups. In vitro culture medium added glycine can significantly enhance somatic cell cloning reorganization of embryo growth rate Yanbian yellow cattle. |
| Keywords: | Yanbian yellow cattle somatic cell cloned glycine |
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