23份中国小麦微核心种质抗叶锈性评价

【目的】探测23份微核心种质材料的抗叶锈性和可能携带的抗叶锈基因。【方法】选取12个具有鉴别能力的小麦叶锈菌生理小种对23份微核心种质进行了苗期和成株期的抗性鉴定以及基因推导，同时结合使用已经报道的能够用于抗病基因分子鉴定的分子标记对其进行进一步抗叶锈基因的分子检测。【结果】这些品种中除中国春表现感病外，其余22份种质均表现出较强的抗性。火球、老齐麦、凤麦11、山红麦、红和尚头、府麦、尕老汉和郑引4号含Lr34和未知抗性基因，中国春含Lr34，碱麦和小佛手含Lr1和Lr34，同家坝小麦和红花麦含Lr34和Lr32，克丰3号含有Lr10、Lr34、Lr16和Lr32，Atlas66含有Lr1、Lr2c和Lr32，烟农15含Lr1，可能含有Lr17，白条鱼含Lr26、Lr16、Lr42和LrZH84，木宗卓嘎含Lr26，可能含Lr14a，金黄麦含Lr1、Lr34和Lr32，云麦34含Lr26、Lr37和LrZH84，可能含有Lr15，百农3217含Lr1和Lr16，白朗灰麦和山麦可能含有未知抗叶锈基因。【结论】这些小麦微核心种质中含有比较丰富的抗叶锈基因，具有较好的抗叶锈性，是抗叶锈育种的重要资源。

Evaluation of Wheat Leaf Rust Resistance of 23 Chinese Wheat Mini-Core Collections

【Objective】The objective of this study was to determine the leaf rust resistance of 23 mini-core wheat accessions, and to detect the Lr genes related to resistance.【Method】 Resistance evaluation and resistance gene postulation of the 23 accessions were carried out with 12 Puccinia triticina races with different virulences at seedling and adult stages. A series of molecular markers linked to wheat leaf rust resistance genes were used for marker test of these accessions.【Result】The 23 accessions from wheat mini-core collections all showed good resistance except Chinese Spring and were postulated to carry some resistance genes. Lr34 and unknown Lr genes in Huoqiu, Laoqimai, Fengmai 11, Shanhongmai, Hongheshangtou, Fumai, Galaohan and Zhengyin 4. Lr34 was found in Chinese Spring. Lr1 and Lr34 were found in Jianmai and Xiaofoshou. Lr34 and Lr32 were identified in Tongjiabaxiaomai and Honghuamai. Lr10, Lr34, Lr16 and Lr32 were postulated in Kefeng 3. Lr1, Lr2c and Lr32 were found in Atlas 66. Lr1 and Lr17 were postulated in Yannong 15. Lr26, Lr16, Lr42 and LrZH84 were found in Baitiaoyu. Lr26 combined with suggested Lr14a in Muzongzhuoga. Lr1, Lr34 and Lr32 were postulated in Jinhuangmai. Lr26, Lr37, LrZH84 and maybe Lr15 were detected in Yunmai 34. Lr1 and Lr16 in Bainong 3217. Besides, Bailanghuimai and Shanmai may carry un-known resistance genes to wheat leaf rust.【Conclusion】The results indicated that the resistance genes in response to leaf rust disease were relatively richer in the 23 Chinese wheat mini-core collections, and the Chinese wheat mini-core collections could be applied in breeding programs for leaf rust resistance.