细胞型朊蛋白在BV2小神经胶质细胞体外激活中的作用

【目的】研究细胞型朊蛋白对小神经胶质细胞不同激活方式的影响。【方法】用IFN-γ、IL-4和IL-10分别刺激BV2细胞，RT-PCR方法检测PrPC的mRNA表达量。SiRNA干扰将PrPC沉默，用上述因子刺激细胞，用RT-PCR和Western-blot检测相关参数。【结果】用IFN-γ、IL-4和IL-10分别刺激小神经胶质细胞后可导致PrPC的mRNA表达量下降；PrPC沉默后的小神经胶质细胞对IFN-γ刺激的反应应答减弱；PrPC沉默可以显著地改变由IL-4诱导的神经胶质细胞的激活表型；但是对IL-10诱导的小神经胶质细胞激活却没有影响。【结论】PrPC既能影响小神经胶质细胞从静止状态到激活状态的转换，也在小神经胶质细胞的经典激活和替代激活途径中发挥调节作用。

Effects of Prion Protein on the Regulation of Classical and Alternative Activation of BV2 Microglia in vitro

【Objective】 The purpose of this study is to investigate the effects of PrPC on various forms of microglial activation. 【Method】 BV2 microglia were treated, respectively, with IFN-γ, IL-4, or IL-10, and the mRNA expression of PRNP was examined by RT-PCR. Then the effects of si-RNA-mediated disruption of PRNP on different parameters of microglial activation in IFN-γ, IL-4, or IL-10-stimulated microglia were analyzed by RT-PCR and western-blot. 【Result】PRNP mRNA expression was invariably downregulated in microglia upon exposure to IFN-γ, IL-4, or IL-10. PRNP silencing prior to cytokines treatment reduced the responsiveness of microglia to INF-γ treatment, significantly altered IL-4-induced microglial activation phenotype, and had no effect on IL-10-induced microglial activation.【Conclusion】Together, these results support a role of PrPC in the modulation of the shift of microglia from a quiescent state to an activated phenotype and in the regulation of the microglial response during classical and alternative activation.