| Ghrelin和GHS-R1a mRNA在牛卵母细胞体外成熟过程中的表达 |
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| 引用本文： | 米焱,张伶俐,李海军,杜晨光,曹贵方.Ghrelin和GHS-R1a mRNA在牛卵母细胞体外成熟过程中的表达[J].中国农业科学,2013,46(9):1939-1945. |
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| 作者姓名： | 米焱 张伶俐 李海军 杜晨光 曹贵方 |
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| 作者单位： | 1.内蒙古农业大学兽医学院，呼和浩特 010018
2.内蒙古农业大学职业技术学院，内蒙古包头 014109 |
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| 基金项目： | 国家自然科学基金（30860201，31060329，31060165）、内蒙古自然科学基金（2012MS0411） |
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| 摘 要： | 【目的】探讨Ghrelin和GHS-R1a mRNA在牛卵母细胞体外成熟过程中的表达及二者表达与FSH之间的关系。【方法】 利用实时定量RT-PCR技术检测体外成熟进程中牛卵母细胞Ghrelin和GHS-R1a mRNA水平表达变化，观察不同浓度FSH对Ghrelin和GHS-R1a mRNA表达的作用以及FSH与FSH受体抑制剂的组合对Ghrelin和GHS-R1a基因表达的影响。【结果】 实时定量RT-PCR结果揭示牛卵母细胞Ghrelin和GHS-R1a mRNA的相对表达量随着成熟过程的延长而呈现一定变化规律，即Ghrelin和GHS-R1a mRNA在卵母细胞体外成熟过程中从8 h开始显著下降并持续此低表达水平到24 h；不同浓度FSH 显著抑制卵母细胞Ghrelin及GHS-R1a mRNA的表达，而FSH 受体抑制剂明显减弱FSH的抑制作用而促进二者表达。【结论】Ghrelin和GHS-R1a mRNA在卵母细胞体外成熟过程中的表达可能随着培养液中FSH升高而降低。
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| 关 键 词： | 牛 卵母细胞 体外成熟 FSH Ghrelin GHS-R1a |
| 收稿时间： | 2012-12-04 |
Expression of Ghrelin and GHS-R1a mRNA in the Bovine Oocytes During in Vitro Maturation |
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| MI Yan,ZHANG Ling-Li,LI Hai-Jun,DU Chen-Guang,CAO Gui-Fang.Expression of Ghrelin and GHS-R1a mRNA in the Bovine Oocytes During in Vitro Maturation[J].Scientia Agricultura Sinica,2013,46(9):1939-1945. |
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| Authors: | MI Yan ZHANG Ling-Li LI Hai-Jun DU Chen-Guang CAO Gui-Fang |
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| Institution: | 1.College of Veterinary, Inner Mongolia Agricultural University, Hohhot 010018;
2.Vocational and Technical College, Inner Mongolia Agricultural University, Baotou 014109, Inner Mongolia |
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| Abstract: | 【Objective】 The aim of this study was to investigate the possibility of Ghrelin and GHS-R1a mRNA expression in bovine oocytes produced in vitro and the relationship between FSH and the expression of Ghrelin and GHS-R1a mRNA. 【Method】Relative real time RT-PCR technology was applied to detect the pattern of Ghrelin and GHS-R1a mRNA expression in oocytes, and the effect of different concentrations FSH and the combination of FSH and FSH binding inhibitor on the expression of Ghrelin and GHS-R1a mRNA were observed. 【Result】 Relative real time RT-PCR experiments confirmed that the Ghrelin and GHS-R1a mRNA levels in oocyte varied depending on the developmental stage, with the significant decrease of Ghrelin and GHS-R1a mRNA expression at 8 h in oocyte maturation process. And addition of FSH inhibited the expression of Ghrelin and GHS-R1a mRNA within oocytes, and this inhibition effect could be attenuated by FSH binding inhibitor. 【Conclusion】 The expression of Ghrelin and GHS-R1a mRNA could be declined with the rising of FSH concentration during in vitro maturation of bovine oocytes. |
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| Keywords: | bovine oocytes in vitro maturation FSH Ghrelin GHS-R1a |
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