陆地棉trihelix转录因子基因响应非生物胁迫表达谱分析

【目的】对陆地棉trihelix转录因子基因在多种胁迫下的应答表达模式进行研究分析，为棉花抗逆相关基因克隆及阐明棉花抗逆分子机制奠定基础。【方法】根据已知24个trihelix转录因子EST序列设计引物，通过RT-PCR和qRT-PCR的方法分析棉花（Gossypium hirsutum L.）GhGT在高盐（200 mmol.L-1NaCl）、干旱、低温（4℃）等非生物胁迫和外源激素ABA（100 μmol.L-1）处理下的表达模式。【结果】12个基因响应高盐和干旱胁迫应答，7个基因响应冷胁迫应答，应答ABA处理的基因9个。此外，有3个基因（GhGT2、GhGT18和GhGT23）在4种处理下均有应答。【结论】GhGTs在陆地棉的非生物胁迫适应过程中可能具有重要的作用。

Expression Profiling of Cotton (Gossypium hirsutum L.) Trihelix Genes Responsive to Abiotic Stresses

【Objective】The objective of this study is to dissect cotton trihelix genes expression alteration responsive to different abiotic stresses, thus providing a foundation for stress-related genes cloning and elucidating the molecular mechanism of cotton stress-resistance.【Method】Semi-quantitative RT-PCR and real-time PCR were employed to reveal the expression profiling of 24 GhGTs under different abiotic stresses (200 mmol.L-1 NaCl, drought, 4℃) and exogenous hormone (100 μmol.L-1ABA) treatments.【Result】The results showed that the expression of 12 GhGT genes were up- and down-regulated by salinity and drought stresses, and that of 7 GhGT genes and 9 GhGT genes were up- and down-regulated by cold and ABA treatments, respectively. Moreover, three genes including GhGT2, GhGT18 and GhGT23 were response to all above treatments. 【Conclusion】The results suggested that GhGTs may play important roles in upland cotton plant abiotic stress adaption.