| 基因工程菌生物合成茶氨酸条件研究 |
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| 引用本文： | 王丽鸳,王贤波,成浩,周健.基因工程菌生物合成茶氨酸条件研究[J].茶叶科学,2007,27(2):111-116. |
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| 作者姓名： | 王丽鸳 王贤波 成浩 周健 |
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| 作者单位： | 1. 农业部茶叶化学工程重点实验室,中国农业科学院茶叶研究所,浙江,杭州,310008
2. 杭州市农业科学院实验中心 |
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| 摘 要： | 研究了不同诱导条件对基因工程菌催化合成茶氨酸的影响,确定了较为优化的诱导表达条件;通过单因子试验研究了不同反应条件对基因工程菌催化合成茶氨酸的影响,研究结果表明反应体系中较高的菌体浓度对催化茶氨酸有一定的抑制作用;高L-Gln浓度可以提高茶氨酸的生成量,但是却降低了L-Gln的转化率;基因工程菌催化合成茶氨酸的最适pH是9.5左右,较为适合的反应温度是32℃~37℃。
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| 关 键 词： | 茶氨酸 基因工程菌 表达 生物合成 |
| 文章编号： | 1000-369X（2007）02-111-06 |
| 收稿时间： | 2006-09-28 |
| 修稿时间： | 2007-02-07 |
Research on Conditions of Theanine Biosynthesis by Genetically Engineered Escherichia coli |
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| WANG Li-yuan,WANG Xian-bo,CHENG Hao,ZHOU Jian.Research on Conditions of Theanine Biosynthesis by Genetically Engineered Escherichia coli[J].Journal of Tea Science,2007,27(2):111-116. |
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| Authors: | WANG Li-yuan WANG Xian-bo CHENG Hao ZHOU Jian |
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| Institution: | Key Lab of Tea Chemical Engineering, Ministry of Agriculture; Tea Research Institute, Chinese Acadc my of Agricultural Sciences, Hangzhou 310008, China |
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| Abstract: | Expression conditions of the genetic engineering E.coli which expressed E.coli γ-GGT were investigated. The optimum IPTG concentration and inductive temperature were determined. Factorial experiment design was employed to determine the effects of the reactive conditions (such as microbe concentration, pH, substitute concentration, reactive temperature) on theanine biosynthesis. Results showed that theanine biosynthesis was inhibited by the high microbe concentration. By the higher concentration of L-Gln, the more theanine was obtained and the convertion rate against Gln was declined. It also was manifested that pH9.5 and 32℃~37℃ were optimum conditions for theanine biosynthsis. |
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| Keywords: | theanine genetically engineered bacteria expression biosynthesis |
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