海洋细菌TC-1对香蕉防御酶活性的影响

为研究海洋生防细菌TC-1对盆栽香蕉防治香蕉枯萎病的诱导抗病机理，通过盆栽试验，不对香蕉苗浇灌TC-1也不接种香蕉枯萎病菌、仅浇灌TC-1、仅接种病原菌和既浇灌TC-1又接种病原菌等4种处理后，测定并比较不同处理的香蕉苗体内5种防御酶活性的差异，探讨生防菌的诱导抗病作用。在未接种香蕉枯萎病菌时，海洋细菌TC-1处理的香蕉苗与未处理的5种防御酶活性没有显著差异（P>0.05）。在接种香蕉枯萎病菌时，用TC-1处理与未处理的对照相比，香蕉苗防御酶活性有显著提高：浇灌TC-1菌液6天后，过氧化物酶和多酚氧化酶活性分别提高了248%和12%；9天后，苯丙氨酸解氨酶和超氧化物歧化酶活性分别提高了16%和10%；12天后，过氧化氢酶活性提高了363%。结果表明菌株TC-1可诱导提高香蕉植株CAT、POD、PPO、PAL、SOD 5种防御酶活性抵抗香蕉枯萎病菌的侵染。

Effect of Antagonistic Marine-born Strain TC-1 on Five Major Defensive Enzymes in Banana

In order to research the inducible resistance mechanism of marine biocontrol bacteria TC-1 against the banana vasieular wilt in the potted trial. The inducible resistance function of TC-1 was explored by comparing the differences of five defense enzymes activities of banana seedling, which were treated via four methods. No treatment was given in normal control group, inducing with fermentation broth of TC-1, inoculating with spore suspension of Fusarium oxysporum f. sp. cubense race 4 （FOC4）, inducing after inoculating with spore suspension of FOC4. The results showed that there were no significant differences on the five kinds of defense enzymes activities in unvaccinated bananas between banana seedlings treated with marine bacteria TC-1 and seedlings with untreated （P〉0.05）. The five kinds of defense enzymes activities were significantly induced in the banana seedlings inoculated FOC4 after treated with TC-1. The activities of POD and PPO in leaf were respectively increased 248% and 12% at 6 day after treatment （DAT）; the activities of PAL and SOD were respectively increased 16% and 10% at 9 DAT. The activity of CAT was respectively increased 363% at 12 DAT simultaneously,, compared to untreated plant. It was concluded that strain TC-1 could played an important role on induction disease resistance to FOC4 by increasing activities of CAT, POD PPO, PAL and SOD in banana plant.