| ctsd、ctsl基因的多态性与雪龙黑牛胴体性状相关性研究 |
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| 引用本文： | 陈晓杰 吴蒙 张立敏 陈翠 刘喜冬 周正奎 张猛 黄萌 刘利 高雪 许尚忠 李俊雅 高会江 张路培 李姣. ctsd、ctsl基因的多态性与雪龙黑牛胴体性状相关性研究[J]. 中国农学通报, 2012, 28(2): 6-9. DOI: 10.11924/j.issn.1000-6850.2011-3158 |
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| 作者姓名： | 陈晓杰 吴蒙 张立敏 陈翠 刘喜冬 周正奎 张猛 黄萌 刘利 高雪 许尚忠 李俊雅 高会江 张路培 李姣 |
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| 作者单位： | [1]中国农业科学院北京畜牧兽医研究所,北京100193 [2]大连雪龙产业集团有限公司,大连116001 |
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| 基金项目： | 农业部专项“现代农业（肉牛）产业技术体系岗位科学家”（CARS-38）;转基因生物新品种培育重大专项“优质高效转基因肉牛新品种培育”（2009ZX08007.005B）;“十一五”转基因重大专项“肉牛脂肪沉积相关基因的转基因研究”（2008ZX08007-2）,国家发改委“国家发改委雪龙黑牛分子育种关键技术研究及产业化应用”. |
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| 摘 要： | 为了提高雪龙黑牛的胴体性状，进行新品种选育，本研究以组织蛋白酶D、L(Cathepsin D, Cathepsin L)基因为候选基因，利用PCR-RFLP方法对114头雪龙黑牛F1代商品牛进行多态性检测，应用SAS 9.2软件采用最小二乘拟合线性模型进行分析，结果表明，ctsd，c581t位点与雪龙黑牛的经济性状无明显的相关性，ctsl基因xma I位点与雪龙黑牛的三角牛腩厚显著相关，且AB型个体三角牛腩厚与AA和BB型个体三角牛腩厚差异显著。
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| 关 键 词： | 基因定位 基因定位 |
| 收稿时间： | 2011-10-28 |
| 修稿时间： | 2011-11-04 |
Genetic Polymorphisms of the CTSD and CTSL Gene and Their Association with Carcass Traits in Snow Dragon black Cattle |
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| Chen Xiaojie,Wu Meng,Zhang Limin,Chen Cui,Liu Xidong,Zhou Zhengkui,Zhang Meng,Huang Meng,Liu Li,Gao Xue,Xu Shangzhong,Li Junya,Gao Huijiang,Zhang Lupei,Li Jiao. Genetic Polymorphisms of the CTSD and CTSL Gene and Their Association with Carcass Traits in Snow Dragon black Cattle[J]. Chinese Agricultural Science Bulletin, 2012, 28(2): 6-9. DOI: 10.11924/j.issn.1000-6850.2011-3158 |
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| Authors: | Chen Xiaojie Wu Meng Zhang Limin Chen Cui Liu Xidong Zhou Zhengkui Zhang Meng Huang Meng Liu Li Gao Xue Xu Shangzhong Li Junya Gao Huijiang Zhang Lupei Li Jiao |
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| Affiliation: | 1.Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193; 2Dalian Snow Dragon Industrial Group Company Ltd., Dalian 116001) |
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| Abstract: | The objective of this study was to explore the genetic polymorphisms of Cathepsin ＇D, L and their association with carcass traits. In this experiment, Genetic polymorphisms of the etsd and ctsl Genes were studied by PCR-RFLE and analyzed their association with meat quality traits in Snow ＇ Dragon black＇ cattle by SAS 9.2. The results showed that etsd, c581t polymorphism had no associated with economic traits, cts1, xma I polymorphism had significantly associated with sirloin triangle thickness （P〈0.05）, sirloin triangle thickness of AB genotype had significant difference with AA and BB genotype. |
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| Keywords: | ＇ Snow Dragon＇ black cattle etsd gene ctsl gene carcass traits |
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