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| 羊草叶片SPAD值与叶绿素含量的相关分析 | |
| 引用本文： | 李辉 白丹 张卓 郭平 周婵 乌兰. 羊草叶片SPAD值与叶绿素含量的相关分析[J]. 中国农学通报, 2012, 28(2): 27-30. DOI: 10.11924/j.issn.1000-6850.2011-1865 |
| 作者姓名： | 李辉 白丹 张卓 郭平 周婵 乌兰 |
| 作者单位： | 1. 辽宁大学生命科学院,沈阳,110036 2. 沈阳大学生物与环境工程学院,沈阳,110044 3. 内蒙古苏尼特右旗草原工作站,内蒙古苏尼特右旗,011200 |
| 基金项目： | 国家自然科学基金项目“羊草叶色渐变群的表型和遗传多样性及其趋异适应机理研究”（30900117）;国家自然科学基金项目“东北草原退化生态系统恢复过程自组织机理研究”（30770397）;辽宁省教育厅科研项目“羊草人工草地繁殖对策的研究”（L2010149）~沈阳市人才资源开发专项资金“优质牧草羊草叶色渐变群种质资源的表型和遗传多样性及高抗逆新品种选育”;辽宁大学“211工程”三期建设项目“现代生物技术与东北种质资源开发利用”. |
| 摘 要： | 本研究采用SPAD叶绿素仪和酒精－丙酮浸提法测定河北沽源和内蒙古图牧集天然草原羊草的叶绿素SPAD值、叶绿素ａ、叶绿素ｂ和总叶绿素含量，探讨野外生态学实验采用叶绿素仪简便快速测定叶绿素的精确度。结果表明，在同一草原不同羊草个体之间羊草叶绿素含量和叶绿素SPAD值在一定范围波动，变异系数为20.457-36.921 %。河北沽源和内蒙古图牧集天然草原羊草叶绿素a、叶绿素b和总叶绿素含量随叶绿素SPAD值的增加而增加，呈线性函数、指数和幂函数规律变化，且均达到极显著相关水平，其中线性函数相关程度最大，可以更准确的预测羊草叶绿素含量。因此，可以利用SPAD-502叶绿素仪在野外条件下原位准确、快速地测定羊草叶绿素，为牧草生产实践提供理论依据 |
| 关 键 词： | 人工种子 人工种子 包埋繁殖体 转株率 存活率 |
| 收稿时间： | 2011-06-23 |
| 修稿时间： | 2011-08-18 |
Correlation analysis between SPAD value and chlorophyll contentof leaf of Leymus chinensis |
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| Li Hui , Bai Dan , Zhang Zhuo , Guo Ping , Zhou Chan , Wu Lan. Correlation analysis between SPAD value and chlorophyll contentof leaf of Leymus chinensis[J]. Chinese Agricultural Science Bulletin, 2012, 28(2): 27-30. DOI: 10.11924/j.issn.1000-6850.2011-1865 | |
| Authors: | Li Hui Bai Dan Zhang Zhuo Guo Ping Zhou Chan Wu Lan |
| Affiliation: | 1 CoUege of Life Science, Liaoning University, Shenyang 110036; 2College of Biology and Environment Engineering, Shenyang University, Shenyang 110044; 3Grassland Station of Sunite Right County, Sunite Right County Inner Mongolia 011200) |
| Abstract: | In order to discuss accuracy of determining chlorophyll with SPAD chlorophyll meter in the eecological field experiments, SPAD value, chlorophyll a, chlorophyll b and total chlorophyll content of Leymus cbinensis on natural grassland were determined with SPAD chlorophyll meter and alcohol-acetone extraction method. The results showed that chlorophyll content and SPAD value of L. cbinensis on different grasslands were changed in the certain range. Coefficient of variation was range from 20.457% to 36.921%. Regression analysis showed that relationships between chlorophyll a content, chlorophyll b content, total chlorophyll content and SPAD value of L. cbinensis on Inner Mongolia Tumuji and Hebei Guyuan natural grassland were significant linear, index and power function. And the coefficient of determination of linear function was highest than those of index and power function. Linear function could greatly reflect the relationships between chlorophyll content and SPAD value of L. chinensis. Chlorophyll content of L. chinensis can be accurately determined on field condition with SPAD chlorophyll meter. This method is simple and fast on field experiment, providing theoretical basis for grass production. |
| Keywords: | Leymus chinensis SPAD value chlorophyll content correlation |
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