| 高活性魔芋葡甘聚糖酶产生菌B23的鉴定及培养条件优化 |
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| 引用本文： | 周海燕,周大寨,周毅峰,吴永尧.高活性魔芋葡甘聚糖酶产生菌B23的鉴定及培养条件优化[J].湖北农业科学,2005(4):67-70. |
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| 作者姓名： | 周海燕 周大寨 周毅峰 吴永尧 |
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| 作者单位： | 1. 湖南农业大学生化与发酵工程实验室,长沙,410128
2. 湖北民族学院生物技术研究所,湖北,恩施,445000 |
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| 基金项目： | 湖北省恩施自治州科技局重点资助项目（EJ012002） |
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| 摘 要： | 葡甘聚糖酶产生菌B23分离自哺乳动物的内脏,经鉴定可能为芽孢杆菌属一新种。该菌生长的最适pH为中性环境,可耐受60℃高温,且对NaCl有很好的耐受力。摇瓶培养时最佳氮源为蛋白胨,魔芋葡甘聚糖做碳源诱导酶的产生,0.2%K2HPO4有利于B23的生长及提高产酶的活性。通过正交试验优化的培养条件为温度40℃、初始pH值7.0～7.2、装液量20%、接种量10%。这些数据为扩大生产葡甘聚糖酶提供了参考。
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| 关 键 词： | 魔芋葡甘聚糖 葡甘聚糖酶 培养条件 细菌鉴定 |
| 文章编号： | 0439-8114(2005)04-0067-04 |
| 收稿时间： | 02 19 2005 12:00AM |
| 修稿时间： | 2005年2月19日 |
Identification of Bacterium B23 Producing High-activity Glucomannanase and Optimization of Its Culture Conditions |
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| ZHOU Hai-yan,ZHOU Da-zhai,ZHOU Yi-feng,WU Yong-yao.Identification of Bacterium B23 Producing High-activity Glucomannanase and Optimization of Its Culture Conditions[J].Hubei Agricultural Sciences,2005(4):67-70. |
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| Authors: | ZHOU Hai-yan ZHOU Da-zhai ZHOU Yi-feng WU Yong-yao |
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| Abstract: | The bacterium producing glulcomannanase named B23 was screened from mammal viscera. By our identification, it was possibly a new species of Bacillus. This bacterium had the optimum pH of neutrality and excellent resistance to heat(60) and hypersaline(10% NaCl). The optimum nitrogen source of rotating culture medium was peptone and carbon source was KGM which could induced the product of glucomannanase. 0.2% K2HPO4 could increase the biomass and the activity of glucomannanase. According to the orthogonal experiment results, The optimized culture condition was temperature 40, initial pH 7.07.2, medium volume 20% and seed culture 10%. The results could provide the glucomannanase production in large scale with references. |
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| Keywords: | konjac glucomannan glucomannanase culture condition bacterial identification |
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