辣椒Capsicum annuum×Capsicum chacoense种间杂种的创制及鉴定

远缘杂交是导入外源有效基因的主要途径之一,种间杂交是拓宽辣椒栽培种遗传基础和创制种质资源的有效方式.为了拓宽栽培辣椒的遗传基础,增强遗传潜力,以辣椒栽培种Capsicum annuum'PI194879'为母本、野生种C.chacoense'PI639651'为父本进行杂交试验,通过形态学观察、花粉育性观察和SSR分子...

Creation and identification of interspecific hybrids between Capsicum annuum and Capsicum chacoense

Distant(Interspecific and Intergeneric)hybridization is an important method for introducing exogenous elite genes into cultivated crops.In pepper,interspecific hybridization is an efficient way to broad genetic basis of cultivated species and develop the excellent germplasm.In order to expand the genetic basis of cultivated pepper and further enhance genetic potential,interspecific hybrid F1(Capsicum annuum×C.chacoense)was created with cultivated species(C.annuum‘PI194879’-female)and wild species(C.chacoense‘PI639651’-male)in this study.To identify the authenticity of interspecific hybrids,analysis of morphological observation,pollen viability and SSR molecular markers were employed on hybrid F1 and their parents.Our results showed that(1)remarkable difference was found in agronomic traits between hybrid F1 and their parents,and majority of phenotypic traits of hybrid F1 presented intermediate values,(2)the pollen viability of interspecific hybrid F1(6.58%)was less than that of female(97.08%)and male(94.51%),and(3)analysis of SSR markers clearly indicated that interspecific hybrid F1 had bands from the paternal and maternal accessions,which confirmed that F1 hybrid was authenticity.Here,interspecific hybrids between C.annuum and C.chacoense will not only expand genetic diversity of cultivated pepper,but also provide the basis for transferring excellent genes from C.chacoense into cultivate pepper.