火焰原子发射光谱法测定鼠尾草属植物中钾和钠的含量

目的]建立发射光谱法测定鼠尾草属植物中K、Na含量的方法。方法]鼠尾草属植物样品采收处理后，80℃消煮至澄清透明后定容。利用火焰原子发射光谱法测定其中K和Na的舍量，优化燃助比、燃烧器高度和分析线等测定因素。结果]经优化，利用火焰原子发射光谱法测定鼠尾草属植物中钾和钠的含量时，助燃气流量分别为1．9和1．8IMmin，燃烧器高度分别为7和8mm，分析线分别为766．5和589．0mm。优化条件下，K、Na的检出限分别为0．0056、0．000600μg/ml，灵敏度分别为0．0245和0．000200μg／ml，加标回收率分别为92．50％～100．50％和95．50％～104．20％；利用火焰原子发射光谱法测定鼠尾草属植物中K和Na的含量相对标准偏差分别为0．44％～3．60％和0～2．90％。结论]火焰原子发射光谱法测定鼠尾草属植物中K、Na含量，操作简便易行，精密度和准确度高，重现性好。

Determination on Potassium and Sodium Contents in Salvia Plants by Using Flame Atomic Emission Spectrophotometry

Objective] The purpose was to establish the determination method of potassium(K)and sodium(Na)in Salvia plants by using emission spectrophotometry.Method] After the Salvia plant samples were collected and treated,they were digested and boiled at 80 ℃ till the solution was clear and transparent and then their volumes were defined.Their K and Na contents were determined by using flame atomic emission spectrophotometry for optimizing the measure factors such as combustion supporting ratio,burner height and analytical line.Result] After optimization,when the K and Na contents in Salvia plants were determined by using flame atomic emission spectrophotometry,the flow rates of combustion and supporting air,burner heights and analytical lines were 1.9 and 1.8 L/min,7 and 8 mm,766.5 and 589.0 nm resp.Under optimized condition,the determination limits,sensitivities and recoveries of standard addition of K and Na were 0.005 6 and 0.006 0 μg/ml,0.024 5 and 0.000 200 μg/ml,92.50%-100.50% and 95.50%-104.20% resp.The RSDs of this method in determining K and Na contents in Salvia plants were 0.44%-3.60% and 0-2.90% resp.Conclusion] The flame atomic emission spectrophotometry was simple,convenient and easy to operate and had high precision and accuracy and good repeatability.