| 一种测定巴西橡胶树胶乳中过氧化氢含量及其在胶乳各组分中分布的方法研究 |
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| 引用本文： | 蔡甫格,王立丰,史敏晶,田维敏. 一种测定巴西橡胶树胶乳中过氧化氢含量及其在胶乳各组分中分布的方法研究[J]. 热带作物学报, 2011, 32(5): 891-894. DOI: 10.3969/j.issn.1000-2561.2011.05.021 |
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| 作者姓名： | 蔡甫格 王立丰 史敏晶 田维敏 |
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| 作者单位： | 1. 海南大学农学院,海南儋州571737;中国热带农业科学院橡胶研究所,农业部橡胶树生物学重点开放实验室,省部共建国家重点实验室培育基地-海南省热带作物栽培生理学重点实验室,海南儋州 571737
2. 中国热带农业科学院橡胶研究所,农业部橡胶树生物学重点开放实验室,省部共建国家重点实验室培育基地-海南省热带作物栽培生理学重点实验室,海南儋州 571737 |
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| 基金项目： | 国家自然科学基金项目（31070535）;现代农业产业技术体系建设专项资金项目（No. CARS-34-GW1）。 |
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| 摘 要： | 胶乳活性氧产生与清除失衡是导致橡胶树发生死皮的一个重要因素。由于直接用丙酮提取胶乳中的过氧化氢,存在测定结果重复性不好和准确性不高的问题。笔者在加入丙酮前使用不同提取液对胶乳样品进行预处理,在加入丙酮后使用萃取剂,建立了一种适合测定胶乳中过氧化氢的方法,发现胶乳中的过氧化氢主要分布于C-乳清和黄色体组分,为进一步研究橡胶树乳管活性氧代谢打下良好基础。
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| 关 键 词： | 橡胶树 胶乳 过氧化氢 C-乳清 黄色体 |
An Improved Technique for Determination of Hydrogen Peroxide in Latex and Its Three Fractions in Rubber Tree(Hevea brasiliensis Muell. Arg.) |
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| CAI Fuge,WANG Lifeng,SHI Minjing and TIAN Weimin. An Improved Technique for Determination of Hydrogen Peroxide in Latex and Its Three Fractions in Rubber Tree(Hevea brasiliensis Muell. Arg.)[J]. Chinese Journal of Tropical Crops, 2011, 32(5): 891-894. DOI: 10.3969/j.issn.1000-2561.2011.05.021 |
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| Authors: | CAI Fuge WANG Lifeng SHI Minjing TIAN Weimin |
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| Affiliation: | 1 College of Agronomy,Hainan University,Danzhou,Hainan 571737,China 2 Ministry of Agriculture Key Laboratory for Rubber Biology,Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737,China |
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| Abstract: | The imbalance between the production and catabolism of reactive oxygen in latex is closely related to the development of tapping panel dryness of rubber tree. The method used to extract H2O2 in latex is defective with respect to repetitive and accuracy. The present study established a suitable method for determining the H2O2 in the latex by pre-treatment with different solutions, followed by using acetone and extractants. Among the three fractions of latex, H2O2 was mainly present in C-serum and lutoids as determined by the improved method. Results could be used as a basis for the investigation of reactive oxygen metabolism in the laticifer of rubber tree. |
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| Keywords: | Hevea brasiliensis Muell. Arg. Latex H2O2 C-serum Lutoid. |
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