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| 槟榔花提取物中没食子酸等9种多酚类化合物的测定 | |
| 引用本文： | 张春梅,黄玉林,程芳芳,王仁才,沈 雁,唐敏敏,陈卫军. 槟榔花提取物中没食子酸等9种多酚类化合物的测定[J]. 热带作物学报, 2011, 32(5): 965-969. DOI: 10.3969/j.issn.1000-2561.2011.05.036 |
| 作者姓名： | 张春梅 黄玉林 程芳芳 王仁才 沈 雁 唐敏敏 陈卫军 |
| 作者单位： | 1. 湖南农业大学园艺园林学院, 湖南长沙 410128;中国热带农业科学院椰子研究所, 海南文昌 571339 2. 中国热带农业科学院椰子研究所,海南文昌,571339 3. 中国热带农业科学院椰子研究所, 海南文昌 571339;桂林理工大学化学与生物工程学院,桂林广西 541004 4. 湖南农业大学园艺园林学院,湖南长沙,410128 5. 中国热带农业科学院椰子研究所, 海南文昌 571339;海南省热带油料作物生物学重点实验室,海南文昌 571339 |
| 基金项目： | 海南省自然科学基金（No. 310101）， 海南省重点科技计划项目（090138）资助。 |
| 摘 要： | 采用反相高效液相色谱-二极管阵列检测法(RP-HPLC-PAD)对槟榔花3种提取物中多酚类化合物的色谱分析条件进行优化,分别探讨流动相的组成、流动相中醋酸浓度、醋酸与甲醇的比例和柱温对保留时间的影响,确定梯度分离条件,并对3种不同槟榔花提取物中多酚类化合物进行定量分析。结果表明,3种提取物中均含没食子酸、香豆酸、表儿茶素、阿魏酸、芦丁和柚皮素,其中表儿茶素、没食子酸和香豆酸的含量相对较高。 |
| 关 键 词： | 反相高效液相色谱-二极管阵列检测法(RP-HPLC-PAD) 槟榔花提取物 多酚 |
Analysis of Nine Phenolic Compounds of Areca Inflorescence Extracts |
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| ZHANG Chunmei,HUANG Yulin,CHENG Fangfang,WANG Rencai,SHEN Yan,TANG Minmin and CHEN Weijun. Analysis of Nine Phenolic Compounds of Areca Inflorescence Extracts[J]. Chinese Journal of Tropical Crops, 2011, 32(5): 965-969. DOI: 10.3969/j.issn.1000-2561.2011.05.036 | |
| Authors: | ZHANG Chunmei HUANG Yulin CHENG Fangfang WANG Rencai SHEN Yan TANG Minmin CHEN Weijun |
| Affiliation: | 2,4 1 College of Landscape and Horticulture,Hunan Agriculture University,Changsha,Hunan 410128,China.2 Institute of Coconut,Chinese Academy of Tropical Agricultural Sciences,Wenchang,Hainan 571339,China 3 College of Chemistry and Bioengineering,Guilin University of Technology,Guilin,Guangxi 541004,China 4 Hainan Key Laboratory for Research of Tropical Palm Plants,Wenchang,Hainan 571339,China |
| Abstract: | The separation conditions of chromatography were optimized with a reversed-phase high performance liquid chromatographic photodiode array detection(RP-HPLC-PAD)method for the determination of nine polyphenols in three Areca inflorescences. The relationship of glacial acetic acid concentration in mobile phase and the retention time, the effects of mobile phase composition, flow phase and the proportion of methanol were discussed respectively. Then the influence of gradient separation conditions was established. At the same time, three Areca inflorescences extracts of phenolic compounds were quantitatively analyzed. The results showed that the three extracts all contained gallic acid, coumalic acid, epicatechin, fumalic acid, naringenin and rutin, in which the contents of epicatechin, gallic acid and coumalic acid were higher than others |
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