| 2个番木瓜多聚半乳糖醛酸酶抑制蛋白基因的克隆与分析 |
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| 引用本文： | 申艳红,陈晓静,李荣荣,卢秉国,何玮毅. 2个番木瓜多聚半乳糖醛酸酶抑制蛋白基因的克隆与分析[J]. 热带作物学报, 2011, 32(5): 861-865. DOI: 10.3969/j.issn.1000-2561.2011.05.015 |
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| 作者姓名： | 申艳红 陈晓静 李荣荣 卢秉国 何玮毅 |
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| 作者单位： | 福建农林大学园艺学院,园艺植物遗传育种研究所,福建福州 350002 |
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| 基金项目： | 福建农林大学校基金资助项目（08B03）;福建省自然科学基金资助项目（2010J05048）。 |
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| 摘 要： | 采用RT-PCR扩增获得了2个番木瓜果实多聚半乳糖醛酸酶抑制蛋白(PGIP)基因cDNA和DNA全长序列,将其命名为CpPGIP1和CpPGIP2.CpPGIP1基因全长为984 bp,编码325个氨基酸;CpPGIP2基因全长为1 025 bp,编码326个氨基酸.2基因均没有内含子序列,核苷酸序列有66.54％的相...
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| 关 键 词： | 番木瓜 多聚半乳糖醛酸酶抑制蛋白(PGIP) 基因克隆 半定量RT-PCR |
Cloning and Analysis of Two Polygalacturonase Inhibiting Protein Genes from Papaya |
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| SHEN Yanhong,CHEN Xiaojing,Li Rongrong,LU Bingguo and HE Weiyi. Cloning and Analysis of Two Polygalacturonase Inhibiting Protein Genes from Papaya[J]. Chinese Journal of Tropical Crops, 2011, 32(5): 861-865. DOI: 10.3969/j.issn.1000-2561.2011.05.015 |
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| Authors: | SHEN Yanhong CHEN Xiaojing Li Rongrong LU Bingguo HE Weiyi |
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| Affiliation: | College of Horticulture, Institute of Genetics & Breeding in Horticultural Plants/Fujian Agriculture and Forestry University;College of Horticulture, Institute of Genetics & Breeding in Horticultural Plants/Fujian Agriculture and Forestry University;College of Horticulture, Institute of Genetics & Breeding in Horticultural Plants/Fujian Agriculture and Forestry University;College of Horticulture, Institute of Genetics & Breeding in Horticultural Plants/Fujian Agriculture and Forestry University;College of Horticulture, Institute of Genetics & Breeding in Horticultural Plants/Fujian Agriculture and Forestry University |
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| Abstract: | |
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| Keywords: | Carica papaya L. Polygalacturonase inhibiting protein Gene clone Semi-quantitative RT-PCR |
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