| 甘蔗叶片宿根矮化病的PCR检测 |
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| 引用本文： | 赵婷婷,王俊刚,杨本鹏,冯翠莲,熊国如,蔡文伟,王文治,伍苏然,张树珍. 甘蔗叶片宿根矮化病的PCR检测[J]. 热带作物学报, 2011, 32(5): 870-873. DOI: 10.3969/j.issn.1000-2561.2011.05.017 |
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| 作者姓名： | 赵婷婷 王俊刚 杨本鹏 冯翠莲 熊国如 蔡文伟 王文治 伍苏然 张树珍 |
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| 作者单位： | 1. 海南大学,海南海口 570228;中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海南海口 571101;中国热带农业科学院甘蔗研究中心,海南海口 571101
2. 中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海南海口 571101;中国热带农业科学院甘蔗研究中心,海南海口 571101 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费(No.ITBBZD0724)，现代农业产业技术体系建设专项基金（No.nycytx-24）。 |
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| 摘 要： | 采用PCR方法对甘蔗植株不同生长时期(幼苗期、分蘖期、拔节期、成熟期)、同一植株的不同叶片及同一叶片的不同部位(叶尖、叶中、叶基)分别取样进行宿根矮化病检测。结果表明:在不同甘蔗生长时期常规PCR方法都可以检测出宿根矮化病阳性植株;对呈阳性植株的不同叶片及同一叶片的不同部位检测均呈阳性,说明被检测甘蔗品种植株不同位置叶片对甘蔗宿根矮化病检测效率影响差异不明显。
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| 关 键 词： | 甘蔗 宿根矮化病 PCR 检测 |
Detection of Leifsonia xyli subsp. xyli in Sugarcane Leaves of Different Growth Stages by Polymerase Chain Reaction |
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| ZHAO Tingting,WANG Jungang,YANG Benpeng,FENG Cuilian,XIONG Guoru,CAI Wenwei,WANG Wenzhi,WU Suran and ZHANG Shuzhen. Detection of Leifsonia xyli subsp. xyli in Sugarcane Leaves of Different Growth Stages by Polymerase Chain Reaction[J]. Chinese Journal of Tropical Crops, 2011, 32(5): 870-873. DOI: 10.3969/j.issn.1000-2561.2011.05.017 |
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| Authors: | ZHAO Tingting WANG Jungang YANG Benpeng FENG Cuilian XIONG Guoru CAI Wenwei WANG Wenzhi WU Suran ZHANG Shuzhen |
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| Affiliation: | 2,3 1 Hainan University,Haikou,Hainan 570228,China 2 Institute of Tropical Bioscience and Biotechnology,CATAS/ Key Biotechnology Laboratory for Tropical Crops,Ministry of Agriculture,Haikou,Hainan 571101,China 3 Svgarcane Research Center CATAS,Haikou,Hainan 571101,China |
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| Abstract: | PCR was used to detect Leifsonia xyli subsp. xyli in sugarcane leaves collected from four growth stages (seedling stage, tillering stage, jointing stage and mature stage), different position of the same plant and three portions of each leaf. The results indicated that leaves from all four growth stages could be diagnosed as positive for L. xyli subsp. xyli infection. In addition, leaves in different positions from L. xyli subsp. xyli positive plants were detected as positive as well. So leaf position didn't affect L. xyli subsp. xyli assay efficiency in the detected sugarcane cultivar. |
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| Keywords: | Sugarcane Ratoon stunting disease PCR Detection |
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