| 龙眼果皮过氧化物酶的分离纯化研究初报 |
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| 引用本文： | 潘东明,林琳,郭志雄. 龙眼果皮过氧化物酶的分离纯化研究初报[J]. 福建农林大学学报(自然科学版), 2003, 32(4): 543-544 |
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| 作者姓名： | 潘东明 林琳 郭志雄 |
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| 作者单位： | 福建农林大学园艺学院,福建,福州,350002 |
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| 基金项目： | 国家"948"计划项目(991029). |
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| 摘 要： | 对福眼龙眼果皮的过氧化物酶(POD)进行分离纯化,得到了一组包含4个不同分子质量的POD.
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| 关 键 词： | 龙眼 过氧化物酶 分离纯化 同工酶 多酚氧化酶 |
| 文章编号： | 1006-7817(2003)04-0543-02 |
| 修稿时间： | 2003-10-09 |
Preliminary study on purification of peroxidase from the peels of longan fruits |
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| PAN Dong-ming,LIN Lin,GUO Zhi-xiong. Preliminary study on purification of peroxidase from the peels of longan fruits[J]. Journal of Fujian Agricultural and Forestry University, 2003, 32(4): 543-544 |
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| Authors: | PAN Dong-ming LIN Lin GUO Zhi-xiong |
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| Abstract: | Peroxidase (POD) was extracted from the peels of matural longan (Dimocarpus longan Lour.cv.Fuyan) fruits. The extraction was precipitated with ammonium sulfate between 30% and 80% saturation. And, the enzyme solution was desalted by Sephadex G-25 column chromatography, separated by DEAE-Cellulose column chromatography, hydroxyapatite (HA) column chromatography and Sephacryl S-200 HR column chromatography respectively. Four fractions of POD whose molecular mass are 290.1, 232.1, 188.5 and 170.7 ku were detected with the silver stain after 5%-20% semi-dry native linear gradient flatted polyacrylamide gel electrophoresis. POD and polyphenol oxidase (PPO) showed no difference in native polyacrylamide gel electrophoresis. Moreover, the purified POD showed the activities of PPO in our preliminary study. |
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| Keywords: | longan peroxidase (POD) purification polyphenol oxidase (PPO) |
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