| 百合叶片离体不定芽发生和快繁技术研究 |
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| 引用本文： | 张艺萍,屈云慧,熊丽,吴学尉.百合叶片离体不定芽发生和快繁技术研究[J].西南农业学报,2006,19(4):751-753. |
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| 作者姓名： | 张艺萍 屈云慧 熊丽 吴学尉 |
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| 作者单位： | 云南省农业科学院花卉研究所,云南,昆明,650205 |
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| 基金项目： | 云南省自然科学基金(2003C0028Q) |
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| 摘 要： | 以百合叶片为材料,建立了百合叶片不定芽发生、植株再生和微繁的技术体系。研究了不同消毒时间、不同激素及其组合对叶片不定芽发生、增殖和生根的效应。结果显示,用0.1%的氯化汞对叶片消毒5 min效果较好,污染率为36%,萌动率为75%,诱导率为68.8%。MS 6-BA 2.0 mg/L NAA 0.5 mg/L是叶片不定芽发生的适宜培养基;MS 6-BA 1.0 mg/L NAA 0.1 mg/L是不定芽增殖的适宜培养基;MS NAA 0.1 mg/L是生根的适宜培养基。
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| 关 键 词： | 百合 叶片 不定芽 快繁 |
| 文章编号： | 1001-4829（2006）04-0751-03 |
| 收稿时间： | 2005-12-02 |
| 修稿时间： | 2005-12-02 |
Research on adventitious shoot regeneration and micropropagation from leaves of lily |
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| ZHANG Yi-ping,QU Yun-hui,XIONG Li,WU Xue-wei.Research on adventitious shoot regeneration and micropropagation from leaves of lily[J].Southwest China Journal of Agricultural Sciences,2006,19(4):751-753. |
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| Authors: | ZHANG Yi-ping QU Yun-hui XIONG Li WU Xue-wei |
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| Institution: | Flower Research Institute of Yunnan Academy of Agricultural Sciences, Yunnan Kunming 650205, China |
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| Abstract: | Adventitious shoot formation,plant regeneration and micro-propagation were established in lily leaf in vitro.Effect of different sterilized time,kinds of hormone and its combination on adventitious shoot formation,plant regeneration and root regeneration were studied.The results showed that explant sterilized in 0.10 % aqueous mercuric chloride for 5 min had better effect,and 36 % of contamination rate,75 % of germinating rate,68.8 % of inducement rate,MS 6-BA 2.0 mg/L NAA 0.5 mg/L was suitable for adventitious shoot formation from the leaf,the shoots grew well in MS 6-BA 1.0 mg/L NAA 0.1 mg/L medium,MS NAA 0.1 mg/L was the best medium for root regeneration. |
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| Keywords: | lily leaves adventitious shoot micro-propagation |
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