| 不同地域柞蚕核型多角体病毒特性的研究 |
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| 引用本文： | 周怀民,谢秉泉,杨明辉,罗怡珊,孙富林.不同地域柞蚕核型多角体病毒特性的研究[J].蚕业科学,1991(3). |
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| 作者姓名： | 周怀民 谢秉泉 杨明辉 罗怡珊 孙富林 |
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| 作者单位： | 河南省云阳蚕业试验场
(周怀民，谢秉泉)，中国科学院武汉病毒研究所
(杨明辉，罗怡珊)，中国科学院武汉病毒研究所(孙富林) |
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| 摘 要： | 探讨了我国不同地域ApNPV的特征性状。试验表明:(1)不同地域的ApNPV对河南柞蚕、蓖麻蚕的致病性有差异;(2)不同地域ApNPV-DNA经EcoRI酶解,电泳图谱相似;使用salI内切酶酶解时,在琼脂糖电泳图谱中的C、G、O、Q、U和P等片段,不同毒株之间有差异;(3)河南云阳株(HY)病毒粒子多肽由16种蛋白亚基组成;病毒DNA为线形环状结构,周长4.76×10~5,计算DNA的分子量为:91.4×10~6道尔顿。
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| 关 键 词： | 柞蚕 核型多角体病毒 病毒性状 |
STUDIES ON THE CHARACTERS OF NUCLER POLYHEDROSIS VIRUS OF ANTHERAEA PERNYI FROM DIFFERENT AREAS IN CHINA |
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| Zhou Huai-min Xie Bing-quan.STUDIES ON THE CHARACTERS OF NUCLER POLYHEDROSIS VIRUS OF ANTHERAEA PERNYI FROM DIFFERENT AREAS IN CHINA[J].Acta Sericologica Sinica,1991(3). |
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| Authors: | Zhou Huai-min Xie Bing-quan |
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| Abstract: | The characters of Antheraea pernyi nuclear polyhedrosis virus (ApNPV) from different areas in China have been studied in this paper. The results showed; ( 1 ) The differences of ApNPV pathogenicities to Henan tussah and eri silkworms vaired with different sources of ApNPV; (2) The electrophoretic patterns were similar among different source ApNPV-DN A digested by EcoRI, but different in C,G,O,Q,U and P fragments when the ApNPV-DNA was digested by endonuclease SalI; (3) The viron of ApNPV from Yunyang consisted of 16 polypeptides with SDS-PAGE. Futhermore,the ApNPV-DNA was confirmed as linear circular form ,4. 76 ×105 A in girth and its molecular weight was 91. 4 ×106 daltons. |
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| Keywords: | Antheraea pernyi Nuclear polyhedrosjs virus Virus character |
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