| 玉米愈伤组织再生体系的研究 |
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| 引用本文： | 徐立华; 周柱华; 许方佐; 阴卫军; 邢燕菊,张举仁. 玉米愈伤组织再生体系的研究[J]. 玉米科学, 2006, 14(6): 096-099 |
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| 作者姓名： | 徐立华 周柱华 许方佐 阴卫军 邢燕菊,张举仁 |
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| 作者单位： | 1. 山东省农业科学院原子能农业应用研究所,济南,250100
2. 山东大学生命科学学院,济南,250100 |
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| 基金项目： | 山东省科技发展计划项目(0310101111)，山东农科院青年基金项目(2005YQ019) |
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| 摘 要： | 用玉米胚性愈伤组织作为转化受体时,不同基因型在相同2,4-D浓度的培养基上诱导,其诱导率有较大差别,齐319最高;胚龄对诱导率的影响也较大,10d左右最易诱导,过大或过小均不易诱导胚性愈伤;培养基中2,4-D的浓度对玉米愈伤组织的诱导率有极大影响,不同基因型的最大胚性愈伤组织诱导率所需的2,4-D浓度也不相同,一般为0.5～1.0mg/L。愈伤组织分化时不同基因型所需最适6-BA浓度不同,齐319、515的愈伤组织最适合分化成苗的6-BA浓度为1mg/L,鲁原341愈伤组织最适6-BA浓度为0.5mg/L。
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| 关 键 词： | 玉米 愈伤组织 诱导率 分化 |
| 文章编号： | 1005-0906（2006）06-0096-04 |
| 收稿时间： | 2006-02-21 |
| 修稿时间： | 2006-02-21 |
Study on the Regenerative System of Maize Calli |
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| XU Li-hua; ZHOU Zhu-hua; XU Fang-zuo; YIN Wei-jun; XING Yan-ju and ZHANG Ju-ren. Study on the Regenerative System of Maize Calli[J]. Journal of Maize Sciences, 2006, 14(6): 096-099 |
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| Authors: | XU Li-hua ZHOU Zhu-hua XU Fang-zuo YIN Wei-jun XING Yan-ju ZHANG Ju-ren |
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| Affiliation: | 1. Institute for Application of A tomic Energy, Shandong Academy of A gricultural Sciences, Jinan 250100; 2. College of Life Science, Shandong University, Jinan 250100, China |
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| Abstract: | Being cultured on the same 2,4-D concentration,calli induction ratios were different among various genotypes,and embryo ages so on.When embryo calli of maize was used to be the receiever in particle-bombardment transformation.It was easy to induce embryo calli about ten days embryo;2,4-D concentration of medium had great efffect on maize calli induction.The great induction ratios of calli on different genotypes need various concentrations of 2,4-D,generally it was from 0.5 to 1.0 mg/L.The properly concentration of 6-BA was different according to various genotypes when calli split up,the suitable concentrations of Q319 and 515 were 1 mg/L,and that of luyuan341 was 0.5 mg/L. |
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| Keywords: | Maize Calli Induction ratios of calli Split up |
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