19个地方鸡种遗传变异的微卫星和AFLP指纹分析

利用20个微卫星标记和6对AFLP引物组合对19个地方鸡种进行了遗传检测,并根据两种标记 分析的遗传距离进行了聚类分析。结果发现,19个地方鸡种在20个微卫星位点上的平均杂合度为0．582 4- 0．743 2,平均多态信息含量为0．523 8-0．702 3;6对AFLP引物组合在19个地方鸡种中共检测到294条多态性 条带,平均每个引物组合产生49条,同时在每个品种群体中还检测到了数量不等的特异性条带;19个地方鸡种两 种标记分析的聚类结果具有较大的一致性,并与所保存地方鸡种的地理分布、现实状况相吻合。这表明同时应用微 卫星和AFLP指纹分析我国地方鸡种的遗传多态性和品种间的亲缘关系,其结果具有更高的可靠性和科学性。

Analysis of Genetic Variation of Chinese Native Chicken Breeds using microsatellite and AFLP Fingerprintings

The 20 microsatellite markers and 6 AFLP primers combinations were used to detect genetic variation of 19 Chinese Native chicken breeds. The results showed that the range of heterozygosity was 0. 582 4--0. 743 2 and the mean polymorphism information content of 20 microsatellite was 0. 523 8-- 0. 702 3. The 6 AFLP primers combinations generated 294 polymorphic bands, 49 polymorphic markers were detected by one primer combination on the average. 1- 15 specific bands were produced in the pool DNA of them. The clustering results of microsatellite were in accordance with that of AFLP and were consistent with the location and present status of 19 chickens. It showed that it was of better credibility to analyze the genetic diversity and relative relationship by using microsatellite and AFLP fingerprinting.