| 猪痢疾密螺旋体水相抗原的提取及鉴定 |
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| 引用本文： | 程国富. 猪痢疾密螺旋体水相抗原的提取及鉴定[J]. 华中农业大学学报, 1994, 13(1): 76-79 |
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| 作者姓名： | 程国富 |
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| 作者单位： | 华中农业大学牧医系 |
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| 摘 要： | 本试验应用热水酚法提取了猪痢疾密螺旋体菌壁的水相抗原，并用琼扩法、火箭电泳法以及被动血凝法测定了该抗原的特异性。结果表明，该抗原能特异性地与兔抗猪痢疾密螺旋体的抗血清和单克隆抗体６Ｈ结合，而与抗非致病性密螺旋体抗血清不发生反应。
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| 关 键 词： | 水相抗原 猪病 痢疾 密螺旋体 |
EXTRACT AND SPECIFICITY OF WATER-PHASE ANTIGEN FROM TREPONEMA HYODYSENTERIAE |
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| Cheng Guofu. EXTRACT AND SPECIFICITY OF WATER-PHASE ANTIGEN FROM TREPONEMA HYODYSENTERIAE[J]. Journal of Huazhong Agricultural University, 1994, 13(1): 76-79 |
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| Authors: | Cheng Guofu |
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| Abstract: | The hot aqueous-phenol method was used for extracting water-phase antigen from Treponema hyodysenteriae. The specificity of the extract was tested with gel-diffusion method,rocket immunoelectrophoresis arid PHA test.The results showed that the extract reacted specifically to anti-Treponema hyodysenteriae antisera and monoclonal antibody(6H),but it did not react to the anti-Treponema innocens antisera. |
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| Keywords: | Treponema hyodysenteriae hot aqueous-phenol method water-phase antigen |
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