| 双灭活原生质体融合构建纤维燃料乙醇全糖发酵高产菌株研究 |
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| 引用本文： | 宋安东,谢慧,王风芹,张建.双灭活原生质体融合构建纤维燃料乙醇全糖发酵高产菌株研究[J].林产化学与工业,2009,29(6):20-24. |
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| 作者姓名： | 宋安东 谢慧 王风芹 张建 |
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| 作者单位： | 河南农业大学生命科学学院,河南郑州,450002 |
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| 基金项目： | 国家农业成果转化资金项目，河南省教育厅2006度高校青年骨干教师资助 |
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| 摘 要： | 以嗜鞣管囊酵母P - 01(Pachusolen tannophilus)和南阳酒精酵母1308(Saccharomyces cerevi-siae)的单倍体D - 12为亲本,通过双灭活原生质体融合技术,获得一株能够进行全糖发酵的稳定的酵母融合株F10,发酵醪液乙醇体积分数达到1.3%,比亲株P - 01的0.95%提高了36.8%.对玉米秸秆水解液进行发酵表明,融合株F10发酵玉米秸秆时乙醇体积分数最高可达1.1%,远高于亲株.
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| 关 键 词： | 双灭活 原生质体融合 全糖发酵 燃料乙醇 |
Construction of Strain Capable of Co-fermenting Pentose and Hexose by Diinactivated Protoplast Fusion for Producing Fuel Ethanol from Cellulose |
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| SONG An-dong,XIE Hui,WANG Feng-qin,ZHANG Jian.Construction of Strain Capable of Co-fermenting Pentose and Hexose by Diinactivated Protoplast Fusion for Producing Fuel Ethanol from Cellulose[J].Chemistry & Industry of Forest Products,2009,29(6):20-24. |
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| Authors: | SONG An-dong XIE Hui WANG Feng-qin ZHANG Jian |
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| Abstract: | A stable fusant F10 which can co-ferment pentose and hexose into fuel ethanol was obtained by diinactivated protoplast fusion between Pachusolen tannophilus P-01 and haploid D-12 of Saccharomyces cerevisiae 1308. The ethanol concentration of F10 in fermentation broth reached 1.3%, or 36.8% higher than parent strain P-01(0.95%). The fermentation activity of F10 in corn-stalk hydrolyzates was analyzed, the ethanol concentration of F10 reached 1.1% (volume fraction), which was far higher than the parent strains. |
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| Keywords: | diinactivation protoplast fusion co-fermenting pentose and hexose fuel ethanol |
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