Molecular analysis and virus transmission tests place Olpidium virulentus, a vector of Mirafiori lettuce big-vein virus and tobacco stunt virus, as a distinct species rather than a strain of Olpidium brassicae |
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Authors: | Takahide Sasaya Hiroki Koganezawa |
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Institution: | (1) National Agricultural Research Center for Western Region, Zentsuji Kagawa, 765-8508, Japan;(2) Kunisada Plant Breeding Station, Kaneko Seeds, Gunma, Japan;(3) Present address: Policy Planning and Evaluation Division, Minister's Secretariat, MAFF, Tokyo, Japan |
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Abstract: | The rDNA-ITS sequences of ten single-sporangium isolates of Olpidium virulentus (a noncrucifer strain of Olpidium brassicae), which transmits Mirafiori lettuce big-vein virus (MLBVV) and tobacco stunt virus (TStV), were compared with those of six single-sporangium isolates of O. brassicae. The sequence similarity within isolates of O. virulentus or O. brassicae was almost identical (98.5%–100.0%), but was low between the two species (79.7%–81.8%). In a phylogenetic analysis of the
rDNA-ITS region, O. virulentus and O. brassicae fell into two distinct clusters, indicating that O. virulentus, a vector of MLBVV and TStV, is a distinct species rather than a strain of O. brassicae. |
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Keywords: | Olpidium virulentus Olpidium brassicae rDNA-ITS Taxonomy |
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