S-RNase genotyping and incompatibility group assignment by PCR and pollination experiments in Japanese plum |
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Authors: | M. E. Guerra,,J. Rodrigo,,M. Ló pez-Corrales, A. Wü nsch |
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Affiliation: | Departamento de Hortofruticultura, Centro de Investigación 'Finca La Orden-Valdesequera', Apdo. 22, 06187 Guadajira, Badajoz;;Unidad de Fruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Avda. Montañana 930, 50059 Zaragoza, Spain;;Corresponding author, E-mail: |
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Abstract: | Most Japanese plum-type cultivars are self-incompatible and cross pollination is necessary to ensure fruit set. In this study, the S -RNase genotype and the incompatibility group of 68 Japanese plum-type cultivars were determined by PCR amplification of the S-RNase gene. The S -RNase genotype of 50 cultivars is first reported here and five new Japanese plum S -RNase alleles ( So , Sp , Sq , Sr , Ss ) were identified. The results obtained, together with information compiled from previous studies, allowed describing 12 new incompatibility groups (VIII–XIX). The self-incompatibility of several cultivars and the cross-compatibility among different incompatibility groups were verified by self- and cross-pollination experiments followed by observation of pollen tube growth. Five cultivars behaved as self-compatible, but two of them do not have the Se allele, which has been correlated with self-compatibility. Thus, additional sources of self-compatibility different from Se appear to be involved in Japanese plum self-compatibility. |
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Keywords: | Prunus salicina S-RNase alleles gametophytic self-incompatibility cross-pollination pollen tube growth pedigree |
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