| 自组装膜电化学免疫传感器测定甲基对硫磷 |
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| 引用本文： | 许园园,吴雅欣,潘家荣. 自组装膜电化学免疫传感器测定甲基对硫磷[J]. 核农学报, 2009, 23(3): 497-500 |
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| 作者姓名： | 许园园 吴雅欣 潘家荣 |
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| 作者单位： | 中国农业科学院农产品加工研究所,农业部农产品加工与质量控制重点实验室,北京,100193 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 利用自组装技术将三巯基丙酸固定在金电极上，以1-乙基-3-（3-二甲氨基）碳二亚胺盐酸盐（EDC）和N-羟基硫代琥珀亚胺（NHS）作为活化剂，将甲基对硫磷抗体以共价键形式固定在三巯基丙酸自组装单分子膜修饰的金电极上，制备了一种免疫传感器。根据抗体与抗原之间特异反应前后电极电位的变化对甲基对硫磷进行定量测定，该传感器的检测下限为0.085 µg/ml，检测范围为0.5~15µg/ml，并可以再生、重复利用。
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| 关 键 词： | 甲基对硫磷 电化学免疫传感器 自组装 |
| 收稿时间： | 2008-09-02 |
SAM Electrochemical Immunosensor in Determination of Methyl Parathion |
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| XU Yuan-yuan,WU Ya-xin,PAN Jia-rong. SAM Electrochemical Immunosensor in Determination of Methyl Parathion[J]. Acta Agriculturae Nucleatae Sinica, 2009, 23(3): 497-500 |
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| Authors: | XU Yuan-yuan WU Ya-xin PAN Jia-rong |
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| Affiliation: | Institute of Agro-Food Science and Technology;CAAS/Key Laboratory of Agriculture Nuclear Technology and Agro-Food Processing;Ministry of Agriculture;Beijing 100193 |
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| Abstract: | An immobilization procedure of antibody on gold electrode is proposed and a SAM(Self-assembled monolayer) electrochemical immunosensor was prepared.A pretreated gold electrode is first immersed in mercaptopropionic acid solution for self assembly through the Au-S covalent bond.Then antibody against methyl pararthion is immobilized to prepare the sensory component.By means of covalent coupling with the activation of 1-ethyl-3-(3-dimethyllaminopropyl) carbodiimide hydrochloride(EDC)) and N-hydroxysulfosuccini... |
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| Keywords: | methyl parathion electrochemical immunosensor self-assembled monolayer |
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